Submitted on October 6, 2004
Revised on December 6, 2004
Accepted on December 20, 2004
Identification of mosquito sterol carrier protein-2 inhibitors
Min-sik Kim, Vilena Wessely, and Que Lan
Entomology, University of Wisconsin-Madison, Madison, WI 53706
Corresponding Author: qlan{at}entomology.wisc.edu
A mosquito sterol carrier protein-2, AeSCP-2, has been shown to aid the uptake of cholesterol in mosquito cells. The discovery of chemical inhibitors of a mosquito sterol carrier protein-2, AeSCP-2, is reported here. AeSCP-2 inhibitors (SCPIs) belong to several chemotypes of hydrophobic compounds. Those inhibitors competed with cholesterol for AeSCP-2 binding with relative high binding affinities. In cultured insect cells, SCPIs reduced cholesterol uptake by as much as 30% at 1-5 uM concentrations. SCPIs were potent larvicides to the yellow fever mosquito, Aedes aegypti, and to the tobacco hornworm, Manduca sexta, with LD50s of 5-21 uM and 0.013-15 ng/mg diet, respectively. The results indicate that a sterol carrier protein-2 has functional similarity in two different insect species.
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