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A more recent version of this article appeared on April 1, 2005

Papers In Press, published online ahead of print January 16, 2005
J. Lipid Res., doi:10.1194/jlr.M400457-JLR200
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Submitted on November 17, 2004
Revised on January 14, 2005
Accepted on January 14, 2005

Characterization of neutral glycolipids of the model filamentous fungus, neurospora crassa: altered expression in plant defensin-resistant mutants

Chaeho Park, Beau Bennion, Isabelle E. J. A. François, Kathelijne K. A. Ferket, Bruno P. A. Cammue, Karin Thevissen, and Steven B. Levery

Department of Chemistry, University of New Hampshire, Durham, NH 03824

Corresponding Author: slevery{at}cisunix.unh.edu

To defend themselves against fungal pathogens, plants produce numerous antifungal proteins and peptides, including defensins, some of which have been proposed to interact with fungal cell surface glycosphingolipid components. Though not known as a phytopathogen, the filamentous fungus Neurospora crassa possesses numerous genes similar to those required for plant pathogenesis identified in fungal pathogens (Galagan et al., 2003, Nature 422:859-868), and it has been used as a model for studying plant-phytopathogen interactions targeting fungal membrane components (Thevissen et al., 2003, Peptides 24:1705-1712). For this study, neutral glycolipid components were extracted from wild type and plant defensin resistant mutant strains of N. crassa. The structures of purified components were elucidated by NMR spectroscopy and mass spectrometry. Neutral glycosphingolipids of both wild type and mutant strains were characterized as beta -glucopyranosylceramides, but those of the mutants were found with structurally altered ceramides. While wild type expressed a preponderance of N-2'- hydroxy-(E)-delta3-octadecenoate as the fatty-N-acyl component attached to the long chain base (4E,8E)-9-methyl-4,8-sphingadienine, the mutant ceramides were found with mainly N-2'-hydroxyhexadecanoate instead. In addition, the mutant strains expressed highly elevated levels of a sterol glucoside identified as ergosterol-beta -glucoside. The potential implications of these findings with respect to defensin resistance in the N. crassa mutants are discussed.


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[Abstract] [Full Text] [PDF]


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