J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on June 1, 2005

Papers In Press, published online ahead of print March 16, 2005
J. Lipid Res., doi:10.1194/jlr.M400481-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M400481-JLR200v1
46/6/1257    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bohlin, K.
Right arrow Articles by Hamvas, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bohlin, K.
Right arrow Articles by Hamvas, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on December 7, 2004
Revised on February 8, 2005
Accepted on March 3, 2005

Metabolic kinetics of pulmonary surfactant in newborn infants using endogenous stable isotope techniques

Kajsa Bohlin, Bruce W. Patterson, Kimberly L. Spence, Assaad Merchak, James C. G. Zozobrado, Luc J. I. Zimmermann, Virgilio P. Carnielli, and Aaron Hamvas

Department of Pediatrics, Washington University, St. Louis, MO 63110

Corresponding Author: hamvas{at}kids.wustl.edu

We compared kinetic indices of pulmonary surfactant metabolism in premature infants (n=41) with respect to (i) tracer ([1-13C1]acetate, [U-13C6]glucose, [1,2,3,4-13C4]palmitate), (ii) phospholipid pool (total phospholipids or disaturated phospholipids), or (iii) instrumentation (gas chromatography/mass spectrometry (GC/MS) or gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS)). Tracer incorporation was measured in phospholipids extracted from serial tracheal aspirates after a 24-hour tracer infusion. The fractional catabolic rate (FCR), representing the total fractional turnover from all sources of surfactant production, was independent of tracer. The fractional synthesis rate of surfactant phospholipid from plasma palmitate was significantly higher than that from palmitate synthesized de novo from acetate, and these two sources of palmitate together accounted for only half of total surfactant production in preterm infants. [U-13C6]glucose showed significant recycling of the 13C-label in intermediary metabolism, distinguishable by GC/MS but not by GC/C/IRMS, resulting in a slower apparent FCR when GC/C/IRMS was used. Extracted phospholipid pool did not impact the surfactant metabolic indices. We suggest that FCR should be used as a primary measure of surfactant turnover kinetics, and that tracers labeling both de novo synthesis (acetate and glucose) and preformed pathways (plasma palmitate) can be used to partition the fractional contribution of each pathway to total production.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 PediatricsHome page
G. Verlato, P. E. Cogo, M. Balzani, A. Gucciardi, I. Burattini, F. De Benedictis, G. Martiri, and V. P. Carnielli
Surfactant Status in Preterm Neonates Recovering From Respiratory Distress Syndrome
Pediatrics, July 1, 2008; 122(1): 102 - 108.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.