J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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A more recent version of this article appeared on August 1, 2005

Papers In Press, published online ahead of print June 1, 2005
J. Lipid Res., doi:10.1194/jlr.M400498-JLR200
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Submitted on December 17, 2004
Revised on May 17, 2005
Accepted on May 23, 2005

Membrane transport of anandamide through resealed human red cell membranes

Inge N. Bojesen and Harald S. Hansen

of Medical Biochemistry and Genetics, Lab. B, University of Copenhagen, Copenhagen DK-2200 N

Corresponding Author: norby{at}imbg.ku.dk

The use of resealed red cell membranes (ghosts) allows the study of transport of a compound in a non-metabolising system with a biological membrane. Transmembrane movements of anandamide(N-arachidonoylethanolamine, arachidonoylethanolamide)have been studied by exchange efflux experiments at 0 C, pH 7.3 with albumin-free and albumin-filled human red cell ghosts. The efflux kinetics is biexponential and is analysed in terms of compartment models. The distribution of anandamide on the membrane inner to outer leaflet pools(B/E)is determined to 0.275+-0.023 and the rate constant of unidirectional flux from inside to outside (k3) is 0.361+-0.023 s-1. The rate constant (km) of unidirectional flux from the membrane to bovine serum albumin in the medium ([BSA]o) increases with the square root of [BSA]o in accordance with the theory of an unstirred layer around ghosts. Anandamide passed through the red cell membrane very rapidly within the order of seconds. At molar ratio of anandamide to bovine serum albumin less than 1, membrane binding of anandamide increases with increasing temperatures between 0 C and 37 C and the equilibrium dissociation constants are in the nM range. The nature of membrane binding and the mechanism of membrane translocation are discussed.


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M. Kaczocha, A. Hermann, S. T. Glaser, I. N. Bojesen, and D. G. Deutsch
Anandamide Uptake Is Consistent with Rate-limited Diffusion and Is Regulated by the Degree of Its Hydrolysis by Fatty Acid Amide Hydrolase
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[Abstract] [Full Text] [PDF]

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 J. Lipid Res.Home page
I. N. Bojesen and H. S. Hansen
Effect of an unstirred layer on the membrane permeability of anandamide
J. Lipid Res., March 1, 2006; 47(3): 561 - 570.
[Abstract] [Full Text] [PDF]


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