J. Lipid Res.
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A more recent version of this article appeared on December 1, 2005

Papers In Press, published online ahead of print September 30, 2005
J. Lipid Res., doi:10.1194/jlr.M500028-JLR200
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Submitted on January 24, 2005
Revised on July 25, 2005
Accepted on September 30, 2005

Transcriptome and proteome analysis of soleus muscle of hormone-sensitive lipase-null mice

Ola Hansson, Morten Donsmark, Charlotte Ling, Pernilla Nevsten, Mikael Danfelter, Jesper L. Andersen, Henrik Galbo, and Cecilia Holm

Department of Experimental Medical Science, Division for Diabetes, Metabolism and Endocrinology, Lund SE 221 84

Corresponding Author: ola.hansson{at}med.lu.se

Hormone-sensitive lipase (HSL), a key enzyme in fatty acid mobilization in adipocytes, has been demonstrated also in skeletal muscle. To gain further insight into the role and importance of HSL in skeletal muscle a transcriptome analysis of soleus muscle of HSL-null mice was performed. 161 transcripts were found to be differentially expressed. Increased mRNA levels of fructose-1,6-bisphosphatase, fructose-2,6-bisphosphatase and phosphorylase kinase gamma 1A suggest a higher glycogen flux in soleus muscle of HSL-null mice. An observed increase in the utilization of glycogen stores supports this finding. Moreover, an increased amount of intramyocellular lipid droplets, observed by transmission electron microscopy, suggests decreased mobilization of lipid stores in HSL-null mice. To complement the transcriptome data a protein expression analysis was performed. Five spots were found to be differentially expressed: pyruvate dehydrogenase E1alpha , creatine kinase, ankyrin-repeat domain 2, glyceraldehyde-3-phosphate dehydrogenase and one protein yet to be identified. Increased protein level of creatine kinase indicates creatine phosphate degradation to be of increased importance in HSL-null mice. The results of this study suggest that in the absence of HSL, a metabolic switch from reliance on lipid to carbohydrate energy substrates takes place, supporting an important role of HSL in soleus muscle lipid metabolism.


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 Am. J. Physiol. Endocrinol. Metab.Home page
C. Fernandez, O. Hansson, P. Nevsten, C. Holm, and C. Klint
Hormone-sensitive lipase is necessary for normal mobilization of lipids during submaximal exercise
Am J Physiol Endocrinol Metab, July 1, 2008; 295(1): E179 - E186.
[Abstract] [Full Text] [PDF]


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