J. Lipid Res.
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A more recent version of this article appeared on July 1, 2005

Papers In Press, published online ahead of print April 16, 2005
J. Lipid Res., doi:10.1194/jlr.M500058-JLR200
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Submitted on February 16, 2005
Revised on April 5, 2005
Accepted on April 8, 2005

Lipid imaging by gold cluster time-of-flight secondary ion mass spectrometry: application to Duchenne muscular dystrophy

David Touboul, Alain Brunelle, Frédéric Halgand, Sabine De La Porte, and Olivier Laprévote

ICSN, CNRS, Gif-sur-Yvette, France 91198

Corresponding Author: Alain.Brunelle{at}icsn.cnrs-gif.fr

Imaging with time-of-flight secondary ion mass spectrometry (TOF-SIMS) has expanded very rapidly with the development of gold cluster ion sources (Au3+). It is now possible to acquire ion density maps (ion images) on a tissue section, without any treatment and with a lateral resolution of few micrometers. In this article, we have taken advantage of this technique to study the degeneration/regeneration process in muscles of a Duchenne Muscular Dystrophy model mouse. Specific distribution of different lipid classes (fatty acids, triglycerids, phospholipids, tocopherol, coenzyme Q9 and cholesterol) allows us to distinguish three different regions on a mouse leg section: one is destructured, while another one is in degeneration (oxidative stress and deregulation of the phosphoinositol cycle) and the last one is stable. TOF SIMS imaging shows here great capabilities to localize directly on a tissue section, a great number of lipid compounds, which reflect the state of the cellular metabolism.


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