Advertisement
J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on October 1, 2005

Papers In Press, published online ahead of print August 1, 2005
J. Lipid Res., doi:10.1194/jlr.M500064-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M500064-JLR200v1
46/10/2134    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, G.
Right arrow Articles by Cook, M. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, G.
Right arrow Articles by Cook, M. E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on February 18, 2005
Revised on July 20, 2005
Accepted on July 27, 2005

10t, 12c-conjugated linoleic acid inhibits lipopolysaccharide-induced cyclooxygenase expression in vitro and in vivo

Guangming Li, David Barnes, Daniel Butz, Dale Bjorling, and Mark E. Cook

University of Wisconsin-Madison, Madison, Wisconsin 53706

Corresponding Author: mcook{at}wisc.edu

Previous data demonstrated CLA reduced eicosanoid release from select organs. We hypothesized that one active CLA isomer was responsible for the reduced prostaglandin release and the mechanism was through the inhibition of inducible cyclooxygenase (COX-2). Here we examined effects of 10t, 12c-CLA and 9c, 11t-CLA on COX-2 protein/mRNA expression, prostaglandin E2 (PGE2) production, and the mechanism by which CLA affects COX-2 expression and prostaglandin release. COX-2 protein expression level was inhibited 80% by 10t, 12c-CLA and 26% by 9c, 11t-CLA at 100 µM in vitro. PGE2 production was decreased from 5.39 ng to 1.12 ng/2×106 cells by 10t, 12c-CLA and from 5.7 ng to 4.5 ng/2×106 cells by 9c, 11t-CLA at 100 µM. Mice fed 10t, 12c-CLA, but not 9c, 11t-CLA were found to have a 34% decrease in COX-2 protein and a 43% reduction of PGE2 release in the lung. 10t, 12c-CLA reduced COX-2 mRNA expression level by 30% at 100 µM in vitro and 30% in mice lung in vivo. Reduced COX-2 mRNA was due to an inhibition of NF-B pathway by 10t, 12c-CLA. The data suggested inhibition of NF-B was one of the mechanisms for the reduced COX-2 expression and PGE2 release by 10t, 12c-CLA.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Nutr.Home page
B. Drury, L. J. Warford-Woolgar, D. J. Herchak, N. Bankovic-Calic, G. Crow, C. G. Taylor, P. Zahradka, M. R. Ogborn, and H. M. Aukema
Dietary trans-10, cis-12 Conjugated Linoleic Acid Reduces Early Glomerular Enlargement and Elevated Renal Cyclooxygenase-2 Levels in Young Obese fa/fa Zucker Rats
J. Nutr., February 1, 2009; 139(2): 285 - 290.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
D. E. Butz, G. Li, S. M. Huebner, and M. E. Cook
A mechanistic approach to understanding conjugated linoleic acid's role in inflammation using murine models of rheumatoid arthritis
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2007; 293(2): R669 - R676.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement