| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
Papers In Press, published online ahead of print June 1, 2005
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
UT Southwestern Medical Center, Dallas, TX 75390-9052
Corresponding Author: jonathan.cohen{at}utsouthwestern.edu
The major pathway for removal of cholesterol from the body is via secretion into the bile. Three members of the ATP binding cassette (ABC) family, ABCG5 (G5), ABCG8 (G8), and ABCB4 (MDR2), are required for efficient biliary export of sterols. Here we examined the interdependence of these three ABC transporters for biliary sterol secretion. Biliary lipid levels in mice expressing no MDR2 (Mdr2-/- mice) were compared to those of Mdr2-/- mice expressing 14 copies of a human (h) G5 and hG8 transgene (Mdr2-/- ;hG5G8Tg mice). Mdr2-/- mice had only trace amounts of biliary cholesterol and phospholipids. The Mdr2-/- ;hG5G8Tg mice had biliary cholesterol levels as low as those of Mdr2-/- mice. Thus, MDR2 expression is required for G5G8-mediated biliary sterol secretion. To determine if the reduction in fractional absorption of dietary sterols associated with G5G8 over-expression is secondary to the associated increase in biliary cholesterol, we compared the fractional absorption of sterols in the Mdr2-/- ;hG5G8Tg and hG5G8Tg animals. Inactivation of MDR2 markedly attenuated the reduction in fractional sterol absorption associated with G5G8 overexpression. These results are consistent with the notion that increased biliary cholesterol secretion contributes to the reduction in fractional sterol absorption associated with G5G8 overexpression.
Revised on May 11, 2005
Accepted on May 20, 2005
ABCG5 and ABCG8 require MDR2 for secretion of cholesterol into bile
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S Kidambi and S B Patel Sitosterolaemia: pathophysiology, clinical presentation and laboratory diagnosis J. Clin. Pathol., May 1, 2008; 61(5): 588 - 594. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. Klaassen and H. Lu Xenobiotic Transporters: Ascribing Function from Gene Knockout and Mutation Studies Toxicol. Sci., February 1, 2008; 101(2): 186 - 196. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Pennings, R. B. Hildebrand, D. Ye, C. Kunne, T. J.C. Van Berkel, A. K. Groen, and M. Van Eck Bone marrow-derived multidrug resistance protein ABCB4 protects against atherosclerotic lesion development in LDL receptor knockout mice Cardiovasc Res, October 1, 2007; 76(1): 175 - 183. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F. Oram and A. M. Vaughan ATP-Binding Cassette Cholesterol Transporters and Cardiovascular Disease Circ. Res., November 10, 2006; 99(10): 1031 - 1043. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kosters, C. Kunne, N. Looije, S. B. Patel, R. P. J. Oude Elferink, and A. K. Groen The mechanism of ABCG5/ABCG8 in biliary cholesterol secretion in mice J. Lipid Res., September 1, 2006; 47(9): 1959 - 1966. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. C. Gelissen, M. Harris, K.-A. Rye, C. Quinn, A. J. Brown, M. Kockx, S. Cartland, M. Packianathan, L. Kritharides, and W. Jessup ABCA1 and ABCG1 Synergize to Mediate Cholesterol Export to ApoA-I Arterioscler. Thromb. Vasc. Biol., March 1, 2006; 26(3): 534 - 540. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
