J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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A more recent version of this article appeared on September 1, 2005

Papers In Press, published online ahead of print June 1, 2005
J. Lipid Res., doi:10.1194/jlr.M500128-JLR200
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Submitted on April 4, 2005
Revised on May 18, 2005
Accepted on May 19, 2005

Simultaneous quantitative determination of deuterium- and carbon-13-labeled essential fatty acids in rat plasma

Yu Hong Lin, Robert J. Pawlosky, and Norman Salem . Jr

LMBB, NIAAA, NIH, Bethesda, MD 20892-9410

Corresponding Author: nsalem{at}niaaa.nih.gov

This study reports methods for the quantitative determination of stable isotope labeled essential fatty acids (EFA) as well as an experiment where deuterium labeled linoleic and a-linolenic acids were compared to those labeled with carbon-13 in rat plasma in vivo. Standard curves were constructed in order to compensate for concentration and plasma matrix effects. It was observed that endogenous pools of fatty acids had a greater suppressing effect on the measurements of 13C-U-labeled EFAs relative to those labeled with 2H5. Using these methods, the in vivo metabolism of orally administered deuterated-linolenate, 13C-U-labeled linolenate, deuterated-linoleate and 13C-U-labeled linoleate was compared in adult rats (n = 11). There were no significant differences in the concentrations of the 2H vs. 13C isotopomers of 18:2n-6, 18:3n-3, 20:4n-6 and 22:6n-3 in rat plasma samples at 24 h after dosing. jlr Thus, there appears to be little isotope effect for 2H5 vs. 13C-U-labeled EFA when the data are calculated using the conventional standard curves and corrected for endogenous fatty acid pool size and matrix effects.


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S. Gagne, S. Crane, Z. Huang, C. S. Li, K. P. Bateman, and J.-F. Levesque
Rapid measurement of deuterium-labeled long-chain fatty acids in plasma by HPLC-ESI-MS
J. Lipid Res., January 1, 2007; 48(1): 252 - 259.
[Abstract] [Full Text] [PDF]

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Y. H. Lin and N. Salem Jr.
In vivo conversion of 18- and 20-C essential fatty acids in rats using the multiple simultaneous stable isotope method
J. Lipid Res., September 1, 2005; 46(9): 1962 - 1973.
[Abstract] [Full Text] [PDF]


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