Advertisement
J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on December 1, 2005

Papers In Press, published online ahead of print September 14, 2005
J. Lipid Res., doi:10.1194/jlr.M500154-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M500154-JLR200v1
46/12/2624    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chang, Y.
Right arrow Articles by Mason, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chang, Y.
Right arrow Articles by Mason, R. J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on April 19, 2005
Revised on September 2, 2005
Accepted on September 8, 2005

KGF induces lipogenic genes through a PI3K and JNK/SREBP-1 pathway in H292 cells

Yongsheng Chang, Jieru Wang, Xiaojun Lu, Douglas P. Thewke, and Robert J. Mason

Medicine Dept., National Jewish Medical and Research Center, Denver, CO 80206

Corresponding Author: warrent{at}njc.org

Lipid synthesis is required for cell growth and is subject to pharmacologic regulation. Keratinocyte growth factor (KGF) stimulates proliferation and lipogenesis in H292 cells, a pulmonary epithelial cancer cell line, but the signaling pathways are not known. KGF stimulated the expression of the transcription factors, SREBP-1, C/EBPalpha , and C/EBPdelta , and two key enzymes involved in lipogenesis, fatty acid synthase (FAS) and stearoyl coenzyme A desaturase 1 (SCD-1). We found that KGF induced rapid activation of Akt, p70S6K, JNK, and ERK. Induction of SREBP-1, SCD-1, and FAS by KGF was inhibited by the JNK inhibitor SP600125 and the PI3 kinase inhibitor LY294002 but not by the ERK inhibitor PD98059. Using FAS and SCD-1-luciferase promoter constructs, we observed that KGF stimulated the transcription of these promoters and that exogenous cholesterol inhibited the induction. Mutation of the SREBP-1 binding site in the SCD-1 promoter abolished the effect of KGF on SCD-1 transcription. In addition, overexpression of active SREBP-1 directly stimulated SCD-1 and FAS. Conversely, adenovirus-mediated overexpression of a dominant negative of SREBP-1 inhibited the KGF effect on FAS and SCD-1 expression. In summary, we conclude that KGF requires both PI3 kinase and JNK signaling pathways to induce SREBP-1, which in turn induces SCD-1, and FAS expression in H292 cells.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Endocr. Rev.Home page
D. Adhikari and K. Liu
Molecular Mechanisms Underlying the Activation of Mammalian Primordial Follicles
Endocr. Rev., August 1, 2009; 30(5): 438 - 464.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Besnard, S. E. Wert, M. T. Stahlman, A. D. Postle, Y. Xu, M. Ikegami, and J. A. Whitsett
Deletion of Scap in Alveolar Type II Cells Influences Lung Lipid Homeostasis and Identifies a Compensatory Role for Pulmonary Lipofibroblasts
J. Biol. Chem., February 6, 2009; 284(6): 4018 - 4030.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
S. Teranishi, K. Kimura, K. Kawamoto, and T. Nishida
Protection of Human Corneal Epithelial Cells from Hypoxia-Induced Disruption of Barrier Function by Keratinocyte Growth Factor
Invest. Ophthalmol. Vis. Sci., June 1, 2008; 49(6): 2432 - 2437.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. Garcia, M. M. Ihrig, R. C. Fry, Y. Feng, S. Xu, S. R. Boutin, A. B. Rogers, S. Muthupalani, L. D. Samson, and J. G. Fox
Genetic Susceptibility to Chronic Hepatitis Is Inherited Codominantly in Helicobacter hepaticus-Infected AB6F1 and B6AF1 Hybrid Male Mice, and Progression to Hepatocellular Carcinoma Is Linked to Hepatic Expression of Lipogenic Genes and Immune Function-Associated Networks
Infect. Immun., May 1, 2008; 76(5): 1866 - 1876.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
E. Furuta, S. K. Pai, R. Zhan, S. Bandyopadhyay, M. Watabe, Y.-Y. Mo, S. Hirota, S. Hosobe, T. Tsukada, K. Miura, et al.
Fatty Acid Synthase Gene Is Up-regulated by Hypoxia via Activation of Akt and Sterol Regulatory Element Binding Protein-1
Cancer Res., February 15, 2008; 68(4): 1003 - 1011.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
R. Qiao, W. Yan, C. Clavijo, R. Mehrian-Shai, Q. Zhong, K.-J. Kim, D. Ann, E. D. Crandall, and Z. Borok
Effects of KGF on Alveolar Epithelial Cell Transdifferentiation Are Mediated by JNK Signaling
Am. J. Respir. Cell Mol. Biol., February 1, 2008; 38(2): 239 - 246.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
V. Besnard, Y. Xu, and J. A. Whitsett
Sterol response element binding protein and thyroid transcription factor-1 (Nkx2.1) regulate Abca3 gene expression
Am J Physiol Lung Cell Mol Physiol, December 1, 2007; 293(6): L1395 - L1405.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Drosatos, D. Sanoudou, K. E. Kypreos, D. Kardassis, and V. I. Zannis
A Dominant Negative Form of the Transcription Factor c-Jun Affects Genes That Have Opposing Effects on Lipid Homeostasis in Mice
J. Biol. Chem., July 6, 2007; 282(27): 19556 - 19564.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
Y. Chang, K. Edeen, X. Lu, M. De Leon, and R. J. Mason
Keratinocyte Growth Factor Induces Lipogenesis in Alveolar Type II Cells through a Sterol Regulatory Element Binding Protein-1c-Dependent Pathway
Am. J. Respir. Cell Mol. Biol., August 1, 2006; 35(2): 268 - 274.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement