J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on November 1, 2005

Papers In Press, published online ahead of print August 16, 2005
J. Lipid Res., doi:10.1194/jlr.M500168-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M500168-JLR200v1
46/11/2388    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yen, C.-L. E.
Right arrow Articles by Farese ., R. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yen, C.-L. E.
Right arrow Articles by Farese ., R. V., Jr
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on May 2, 2005
Revised on July 29, 2005
Accepted on August 3, 2005

A human skin multifunctional O-acyltransferase that catalyzes the synthesis of acylglycerols, waxes, and retinyl esters

Chi-Liang Eric Yen, Charles H. Brown IV., Mara Monetti, and Robert V. Farese . Jr

Gladstone Insitute of Cardiovascular Disease, Gladstone Institutes, UCSF, San Francisco, CA 94158

Corresponding Author: eyen{at}gladstone.ucsf.edu

Acyl CoA-dependent O-acyltransferases catalyze reactions in which fatty acyl CoAs are joined to acyl acceptors containing free hydroxyl groups to produce neutral lipids. In this report, we characterize a human multifunctional O-acyltransferase (designated MFAT) that belongs to the DGAT2/MGAT gene family and is highly expressed in the skin. Membranes of insect cells and homogenates of mammalian cells overexpressing MFAT exhibited significantly increased acyl CoA:monoacylglycerol acyltransferase (MGAT), acyl CoA:fatty acyl alcohol acyltransferase (wax synthase), and acyl CoA:retinol acyltransferase (ARAT) activities, which catalyze the synthesis of diacylglycerols, wax monoesters, and retinyl esters, respectively. Furthermore, when provided with the appropriate substrates, intact mammalian cells overexpressing MFAT accumulated more waxes and retinyl esters than did control cells. We conclude that MFAT is a multifunctional acyltransferase that likely plays an important role in lipid metabolism in human skin.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Lipid Res.Home page
C.-L. E. Yen, S. J. Stone, S. Koliwad, C. Harris, and R. V. Farese Jr.
Thematic Review Series: Glycerolipids. DGAT enzymes and triacylglycerol biosynthesis
J. Lipid Res., November 1, 2008; 49(11): 2283 - 2301.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Lardizabal, R. Effertz, C. Levering, J. Mai, M.C. Pedroso, T. Jury, E. Aasen, K. Gruys, and K. Bennett
Expression of Umbelopsis ramanniana DGAT2A in Seed Increases Oil in Soybean
Plant Physiology, September 1, 2008; 148(1): 89 - 96.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Wongsiriroj, R. Piantedosi, K. Palczewski, I. J. Goldberg, T. P. Johnston, E. Li, and W. S. Blaner
The Molecular Basis of Retinoid Absorption: A Genetic Dissection
J. Biol. Chem., May 16, 2008; 283(20): 13510 - 13519.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
F. Beisson, Y. Li, G. Bonaventure, M. Pollard, and J. B. Ohlrogge
The Acyltransferase GPAT5 Is Required for the Synthesis of Suberin in Seed Coat and Root of Arabidopsis
PLANT CELL, January 1, 2007; 19(1): 351 - 368.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. J. Stone, M. C. Levin, and R. V. Farese Jr.
Membrane Topology and Identification of Key Functional Amino Acid Residues of Murine Acyl-CoA:Diacylglycerol Acyltransferase-2
J. Biol. Chem., December 29, 2006; 281(52): 40273 - 40282.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.