| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
Papers In Press, published online ahead of print October 12, 2005
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Lipid Research Group, The Heart Research Institute, Camperdown, New South Wales 2050
Corresponding Author: karye{at}ozemail.com.au
The HDL in human plasma are classified on the basis of apolipoprotein composition into those that contain apolipoprotein A-I (apoA-I) without apoA-II, (A-I)HDL, and those containing apoA-I and apoA-II, (A-I/A-II)HDL. ApoA-I is synthesized in the liver and intestine, and secreted into the plasma as discoidal particles complexed with phospholipids. Discoidal (A-I)HDL are remodelled into spherical (A-I)HDL by LCAT. ApoA-II is synthesized in the liver, and is probably also secreted into the plasma as a component of discoidal HDL. As discoidal HDL that contain apoA-II, (A-II)HDL, are poor substrates for LCAT, they are not converted into spherical (A-II)HDL. This study investigates the fate of apoA-II that enters the plasma in either the lipid-free form or as a component of discoidal (A-II)HDL. Lipid-free apoA-II, and apoA-II-containing discoidal reconstituted HDL, (A-II)rHDL, were injected intravenously into New Zealand White rabbits, a species that is naturally deficient in apoA-II, and does not have endogenous (A-I/A-II)HDL. In both cases the apoA-II was rapidly and quantitatively incorporated into spherical HDL that also contained apoA-I to form spherical (A-I/A-II)HDL. These particles were comparable in size and composition to the spherical (A-I/A-II)HDL in human plasma. Injection of lipid-free apoA-II and discoidal (A-II)rHDL into rabbits also resulted in triglyceride-enrichment of the endogenous (A-I)HDL and VLDL, as well as the newly formed (A-I/A-II)HDL. It is concluded that, irrespective of whether apoA-II enters the plasma in the lipid-free form or as a component of discoidal (A-II)rHDL, it is rapidly incorporated into spherical HDL that also contain apoA-I to form (A-I/A-II)HDL.
Revised on September 29, 2005
Accepted on October 11, 2005
Formation of high density lipoproteins containing both apolipoprotein A-I and A-II in the rabbit
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  X. Moren, S. Deakin, M.-L. Liu, M.-R. Taskinen, and R. W. James HDL subfraction distribution of paraoxonase-1 and its relevance to enzyme activity and resistance to oxidative stress J. Lipid Res., June 1, 2008; 49(6): 1246 - 1253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. W. Castellani, C. N. Nguyen, S. Charugundla, M. M. Weinstein, C. X. Doan, W. S. Blaner, N. Wongsiriroj, and A. J. Lusis Apolipoprotein AII Is a Regulator of Very Low Density Lipoprotein Metabolism and Insulin Resistance J. Biol. Chem., April 25, 2008; 283(17): 11633 - 11644. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. G. D. Silva, L. A. Schneeweis, S. C. Krishnan, X. Zhang, P. H. Axelsen, and W. S. Davidson The Structure of Apolipoprotein A-II in Discoidal High Density Lipoproteins J. Biol. Chem., March 30, 2007; 282(13): 9713 - 9721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Dugue-Pujol, X. Rousset, D. Pastier, N. T. Quang, V. Pautre, J. Chambaz, M. Chabert, and A.-D. Kalopissis Human apolipoprotein A-II associates with triglyceride-rich lipoproteins in plasma and impairs their catabolism J. Lipid Res., December 1, 2006; 47(12): 2631 - 2639. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
