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Papers In Press, published online ahead of print January 28, 2006
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Biochemistry Dept., Boston Univ. School of Medicine, Boston, MA 02118-2526
Corresponding Author: cecmsms{at}bu.edu
A simple and robust LC-MS based methodology for the investigation of lipid mixtures is described and its application to the analysis of human lipoprotein-associated lipids is demonstrated. After an optional initial fractionation on Silica 60, normal-phase HPLC-MS on a YMC PVA-Sil column is employed first for class separation, followed by reversed-phase LC-MS or LC-MS/MS using an Atlantis dC18 capillary column, and/or nanospray MS, to fully characterize the individual lipids. The methodology is applied here for the analysis of human apolipoprotein B-associated lipids. This approach allows for the determination of even low percentages of lipids of each molecular species, and showed clear differences between lipids associated with apolipoprotein B100-LDL isolated from a normal individual and those associated with a truncated version, apolipoprotein B67-containing lipoproteins, isolated from a homozygote patient with familial hypobetalipoproteinemia. The methods described should be easily adaptable to most modern MS instrumentation.
Revised on January 24, 2006
Accepted on January 28, 2006
LC-MS based method for the qualitative and quantitative analysis of complex lipid mixtures
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