J. Lipid Res.
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A more recent version of this article appeared on January 1, 2007

Papers In Press, published online ahead of print October 21, 2006
J. Lipid Res., doi:10.1194/jlr.M600155-JLR200
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Submitted on April 4, 2006
Revised on September 26, 2006
Accepted on October 21, 2006

Temporal and spatial variations of lipid droplets during adipocyte division and differentiation

Masafumi Nagayama, Tsutomu Uchida, and Kazutoshi Gohara

Division of Applied Physics, Graduate School of Engineering, Hokkaido University, Sapporo 060-8628

Corresponding Author: macci{at}eng.hokudai.ac.jp

By capturing time-lapse images of primary stromal-vascular cells (SVCs) derived from rat mesenteric adipose tissue, we revealed temporal and spatial variations of lipid droplets (LDs) in individual SVCs during adipocyte differentiation. Numerous small LDs (a few micrometers in diameter) appeared in the perinuclear region at an early stage of differentiation; subsequently, several LDs grew to more than 10 µm in diameter and occupied the cytoplasm. We have developed a method for the fluorescence staining of LDs in living adipocytes. Time-lapse observation of the stained cells at higher magnification showed that nascent LDs (several 100 nm in diameter) grew into small LDs while moving from lamellipodia to the perinuclear region. We also found that adipocytes are capable of division and they evenly distribute the LDs between two daughter cells. Immunofluorescence observations of lipid droplet-associated proteins revealed that such cell divisions of SVCs occurred even after LDs were coated with perilipin, suggesting that the “final” cell division during adipocyte differentiation occurs considerably later than that characterized in 3T3-L1 cells. Our time-lapse observations have provided a detailed account of the morphological changes that SVCs undergo during adipocyte division and differentiation.


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