Submitted on May 15, 2006
Revised on June 29, 2006
Accepted on June 29, 2006
The age-related paraoxonase 1 response is altered by long-term caloric restriction in male and female rats
Elena Thomàs-Moyà, Magdalena Gianotti, Ana M. Proenza, and Isabel Lladó
Biologia Fonamental i Ciències de la Salut, Universitat de les Illes Balears, Palma de Mallorca, Illes Balears 07122
Corresponding Author: isabel.llado{at}uib.es
Caloric restriction (CR) has been shown to attenuate age-related oxidative damage and to improve major atherosclerotic risk factors. Paraoxonase 1 (PON1), an enzyme specifically associated to HDL containing apolipoproteins A-I and J, has been reported to prevent the proatherosclerotic effects of oxidized LDL. The aim of this study was to evaluate whether modulation of PON1 activity is part of the underlying CR mechanisms that attenuate the age-associated negative effects. Experimental groups were one-year old rats of both genders subjected to 40% CR for one year, and two ad libitum fed groups –also including rats of both genders– sacrificed at six months or two years. Aging impairs the serum lipid profile and increases lipid peroxidation, PON1 activities and the content of both PON1 and apolipoprotein J in HDL, which suggests an HDL subfraction redistribution to protect LDL more effectively from oxidation. The CR-associated improved lipid profile and the decreased lipid peroxide levels would lead to the decreased arylesterase activity seen in old CR animals, thus suggesting that PON1 modulation is not an integral part of the main antioxidant mechanisms of CR, but rather CR would determine a more youthful and less oxidative situation in which protection of LDL would be less necessary.
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