Advertisement
J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on October 1, 2006

Papers In Press, published online ahead of print July 17, 2006
J. Lipid Res., doi:10.1194/jlr.M600269-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M600269-JLR200v1
47/10/2306    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Khan, N. A.
Right arrow Articles by Igarashi, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Khan, N. A.
Right arrow Articles by Igarashi, K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on June 20, 2006
Revised on July 17, 2006
Accepted on July 17, 2006

Docosahexaenoic acid inhibits growth of mouse mammary cancer cells by interfering with p27/kip1, Cdk2, ERK1/ERK2 and retinoblastoma phosphorylation

Naim A. Khan, Kazuhiro Nishimura, Virginie Aires, Tomoko Yamashita, David Oaxaca-Castillo, Keiko Kashiwagi, and Kazuei Igarashi

Physiologie, Université de Bourgogne, Dijon 21000

Corresponding Author: Naim.Khan{at}u-bourgogne.fr

Docosahexaenoic acid (DHA), a polyunsaturated fatty acid of n-3 family, inhibited growth of FM3A mouse mammary cancer cells by arresting their progression from late-G1 to S phase of cell cycle. DHA upregulated p27Kip1 levels by inhibiting phosphorylation of mitogen-activated protein (MAP) kinases, i.e., ERK1/ERK2. Indeed, inhibition of ERK1/ERK2 phosphorylation by DHA, U0126 (chemical MEK inhibitor) and MEKSA (cells expressing dominant negative constructs of MEK) resulted in the accumulation of p27Kip1. MAPK inhibition by DHA did not increase p27Kip1 mRNA levels rather this fatty acid stabilized p27Kip1 contents and inhibited MAPK-dependent proteasomal degradation of this protein. DHA also diminished cyclin E phosphorylation, cyclin-dependent kinase 2 (CDK2) activity and phosphorylation of retinoblastoma protein (pRb) in these cells. Our study shows that DHA arrests cell growth by modulating the phosphorylation of cell cycle-related proteins.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 FASEB J.Home page
H. Gleissman, R. Yang, K. Martinod, M. Lindskog, C. N. Serhan, J. I. Johnsen, and P. Kogner
Docosahexaenoic acid metabolome in neural tumors: identification of cytotoxic intermediates
FASEB J, March 1, 2010; 24(3): 906 - 915.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
A. Yessoufou, A. Ple, K. Moutairou, A. Hichami, and N. A. Khan
Docosahexaenoic acid reduces suppressive and migratory functions of CD4CD25 regulatory T-cells
J. Lipid Res., December 1, 2009; 50(12): 2377 - 2388.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
R. Gorjao, S. M. Hirabara, T. M. de Lima, M. F. Cury-Boaventura, and R. Curi
Regulation of interleukin-2 signaling by fatty acids in human lymphocytes
J. Lipid Res., September 1, 2007; 48(9): 2009 - 2019.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
S. Lopez, B. Bermudez, Y. M. Pacheco, G. Lopez-Lluch, W. Moreda, J. Villar, R. Abia, and F. J. G. Muriana
Dietary Oleic and Palmitic Acids Modulate the Ratio of Triacylglycerols to Cholesterol in Postprandial Triacylglycerol-Rich Lipoproteins in Men and Cell Viability and Cycling in Human Monocytes
J. Nutr., September 1, 2007; 137(9): 1999 - 2005.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2006 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement