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Papers In Press, published online ahead of print January 8, 2007
J. Lipid Res., doi:10.1194/jlr.M600413-JLR200
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Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390
Corresponding Author: richard.anderson{at}utsouthwestern.edu
Lipid droplets are accumulations of neutral lipids surrounded by a monolayer of phospholipids and associated proteins. Recent proteomic analysis of isolated droplets suggests they are part of a dynamic organelle system that is involved in membrane traffic as well as packaging and distributing lipids in the cell. To gain a better insight into the function of droplets, we have used a combination of mass spectrometry and NMR spectroscopy to characterize the lipid composition of this compartment. In addition to cholesterol esters and triacylglycerols with mixed fatty acid composition, we found that about 10-20% of the neutral lipids were the ether lipid, monoalk(en)yl diacylglycerol. Although lipid droplets contain only 1-2% phospholipids by weight, more than 160 molecular species were identified and quantified. Phosphatidylcholine (PC) was the most abundant class followed by phosphatidylethanolamine (PE), phosphatidylinositol (PI) and ether-linked phosphatidylcholine (ePC). Relative to total membrane, droplet phospholipids were enriched in lysoPE, lysoPC, and PC but deficient in sphingomyelin, phosphatidylserine and phosphatidic acid. These results suggest that droplets play a central role in ether lipid metabolism and intracellular lipid traffic.
Revised on January 8, 2007
Accepted on January 8, 2007
Lipidomics reveals adiposomes store ether lipids and mediate phospholipid traffic
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