J. Lipid Res.
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A more recent version of this article appeared on August 1, 2007

Papers In Press, published online ahead of print May 11, 2007
J. Lipid Res., doi:10.1194/jlr.M700131-JLR200
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Submitted on March 19, 2007
Accepted on May 11, 2007

Direct evidence of lipid translocation between adipocytes and prostate cancer cells with imaging FTIR microspectroscopy

Ehsan Gazi, Peter Gardner, Nicholas P. Lockyer, Claire A. Hart, Michael D. Brown, and Noel W. Clarke

ProMPT Genito Urinary Cancer Research Group, Paterson Institute for Cancer Research, Manchester M20 4BX

Corresponding Author: Egazi{at}picr.man.ac.uk

Various epidemiological studies show a positive correlation between high intake of dietary fatty acids (FAs) and metastatic prostate cancer (CaP). Moreover, CaP metastasises to the bone marrow, which harbours a rich source of lipids stored within adipocytes. Here, we use FTIR microspectroscopy to study adipocyte biochemistry and to demonstrate that PC-3 cells uptake isotopically labelled FA (deuterated palmitic acid (D31-PA)), from an adipocyte. Using this vibrational spectroscopic technique we detected subcellular locations in a single adipocyte enriched with (D31-PA) using the upsilon as+s(C-D)2+3 (D31-PA) : upsilon {as+s(C-H) 2+3 (lipid hydrocarbon) signal. In addition, larger adipocytes were found to consist of a higher percentage of (D31-PA) of the total lipid found within the adipocyte. Following background subtraction, the upsilon {as+s(C-D)2+3 signal illuminated starved PC-3 cells co-cultured with (D31-PA) loaded adipocytes, indicating translocation of the labelled FA. This study demonstrates lipid-specific translocation between adipocytes and tumour cells and the use of FTIR microspectroscopy to characterise various biomolecular features of a single adipocyte without the requirement for cell isolation and lipid extraction.


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