Elevated concentrations of circulating vitamin E in carriers of the apolipoprotein A5 gene -1131T>C variant and associations with plasma lipids and lipid peroxidation

Isabella Sundl, Montse Guardiola, Gholamali Khoschsorur, Rosa Solà, Joan C. Vallvé, Gemma Godàs, Lluís Masana, Michaela Maritschnegg, Andreas Meinitzer, Nicolas Cardinault, Johannes M. Roob, Edmond Rock, Brigitte M. Winklhofer-Roob, and Josep Ribalta

Universitat Rovira i Virgili, Unitat de Recerca de Lípids i Arteriosclerosi, Facultat de Medicina, Reus 43201

Corresponding Author: josep.ribalta{at}urv.cat

The aim of this study was to investigate the effects of the apolipoprotein A5 (APOA5) 1131T>C gene variant on vitamin E status and lipid profile. The gene variant was determined in 297 healthy non-smoking men aged 20-75 years and recruited in the VITAGE-project. Effects of the genotype on vitamin E in plasma, low density lipoproteins (LDL) and buccal mucosal cells (BMC) as well as on cholesterol and triglyceride concentrations in plasma and lipoproteins, apo A-I, B, E, C-III and plasma fatty acids were determined. Plasma malondialdehyde concentrations as a marker of in vivo lipid peroxidation were determined. C allele carriers showed significantly higher triglycerides, VLDL and LDL in plasma, higher cholesterol in VLDL and intermediate density lipoprotein (IDL), as well as higher plasma fatty acids. Plasma a-tocopherol (but neither -tocopherol, LDL a- and -tocopherol, nor BMC total vitamin E) was significantly increased in C allele carriers as compared to homozygote T allele carriers (P=0.02), but not after adjustment for cholesterol or triglycerides. Plasma malondialdehyde concentrations did not differ between genotypes. In conclusion, higher plasma lipids in the TC+CC genotype are efficiently protected against lipid peroxidation by higher a-tocopherol concentrations. Lipid-standardized vitamin E should be used for reliably assessing vitamin E status in genetic association studies.

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