J. Lipid Res.
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A more recent version of this article appeared on February 1, 2008

Papers In Press, published online ahead of print November 17, 2007
J. Lipid Res., doi:10.1194/jlr.M700421-JLR200
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Submitted on September 24, 2007
Revised on November 14, 2007
Accepted on November 17, 2007

Lipid mapping in human dystrophic muscle by cluster-time-of-flight secondary ion mass spectrometry imaging

Nora Tahallah, Alain Brunelle, Sabine De La Porte, and Olivier Laprévote

ICSN, CNRS, Gif-sur-Yvette, France 91198

Corresponding Author: Olivier.Laprevote{at}icsn.cnrs-gif.fr

Human striated muscle samples, from male control and Duchenne Muscular Dystrophy (DMD)-affected children, have been subjected to cluster-time-of-flight secondary ion mass spectrometry (cluster-ToF-SIMS) imaging, using a 25 keV Bi3+ liquid metal ion gun, under static SIMS conditions. Spectra and ion density maps, or secondary ion images, have been acquired in both positive and negative ion mode over several areas of 500 x 500 µm2 (image resolution 256 x 256 pixels). Characteristic distributions of various lipids have been observed. Vitamin E and phosphatidylinositols have been found to concentrate within the cells, whereas intact phosphocholines accumulated over the most damaged areas of the dystrophic muscles, together with cholesterol and sphingomyelin species. Fatty acyl chains composition varied depending on the region, allowing estimation of the local damage extent.


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