Advertisement
J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on October 1, 2008

Papers In Press, published online ahead of print July 3, 2008
J. Lipid Res., doi:10.1194/jlr.M700600-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M700600-JLR200v1
49/10/2124    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yee, J. K.
Right arrow Articles by Lee, W.-N. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yee, J. K.
Right arrow Articles by Lee, W.-N. P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on December 27, 2007
Revised on July 2, 2008
Accepted on July 2, 2008

Compartmentalization of stearoyl-CoA desaturase enzyme-1 activity in HepG2 cells

Jennifer K. Yee, Catherine S. Mao, Heidi S. Hummel, Shu Lim, Sharon Sugano, Virender K. Rehan, Gary Xiao, and Wai-Nang P. Lee

Pediatrics, Los Angeles Biomedical Research Institute at Harbor-UCLA, Torrance, CA 90509

Corresponding Author: lee{at}labiomed.org

Stearoyl-CoA desaturase 1 (SCD1) catalyzes conversion of stearate (18:0) to oleate (18:1n-9), and palmitate (16:0) to palmitoleate (16:1), which are key steps in triglyceride synthesis in the fatty acid metabolic network. This study investigated the role of SCD1 in fatty acid metabolism in HepG2 cells using SCD1 inhibitors and stable isotope tracers. HepG2 cells were cultured with [U13C]-stearate or [U13C]-palmitate, [1, 2 13C]-acetate, and 1) DMSO, or 2) compound CGX0168 or CGX0290, or 3) trans-10, cis-12 conjugated linoleic acid (CLA). 13C incorporation into fatty acids was determined by GC/MS and desaturation indices calculated from the respective ion-chromatograms. FAS, SCD1, PPARa, and PPAR mRNA levels were assessed by semi-quantitative reverse transcriptase-PCR. The addition of CGX0168 and CGX0290 decreased the stearate and palmitate desaturation indices in HepG2 cells. CLA led to a decrease in the desaturation of stearate only, but not palmitate. Comparison of desaturation indices based on isotope enrichment ratios differed, depending on the origin of saturated fatty acid. SCD1 gene expression was not affected in any group. In conclusion, the differential effects of SCD1 inhibitors and CLA on SCD1 activity combined with the dependence of desaturation indices on the source of saturated fatty acid strongly support compartmentalization of desaturation systems. Effects of SCD1 inhibition on fatty acid composition in HepG2 cells occurred through changes in the dynamics of the fatty acid metabolic network and not through transcriptional regulatory mechanisms.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2008 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement