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J. Lipid Res.
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A more recent version of this article appeared on May 1, 2009

Papers In Press, published online ahead of print January 9, 2009
J. Lipid Res., doi:10.1194/jlr.M800658-JLR200
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Submitted on December 19, 2008
Revised on January 6, 2009
Accepted on January 8, 2009

Mitochondria do not contain lipid rafts and lipid rafts do not contain mitochondrial proteins

Yu Zi Zheng, Kyra B. Berg, and Leonard J. Foster

Centre for High-Throughput Biology, Department of Biochemistry & Molecular Biology, University of British Columbia, Vancouver, BC V6T 1Z4

Corresponding Author: ljfoster{at}interchange.ubc.ca

Lipid rafts are membrane microdomains involved in many cellular functions, including transduction of cellular signals and cell entry by pathogens. Lipid rafts can be enriched biochemically by extraction in a non-ionic detergent at low temperature, followed by floatation on a sucrose density gradient. Previous proteomic studies of such detergent-resistant membranes (DRMs) are in disagreement about the presence of mitochondrial proteins in raft components. Here we approach the status of mitochondrial proteins in DRM preparations by employing Stable Isotope Labeling by Amino acids in Cell culture (SILAC) to evaluate the composition of differentially purified subcellular fractions, as well as high-resolution linear density gradients. Our data demonstrate that F1/F0 ATPase subunits, voltage-dependent anion selective channels (VDACs) and other mitochondrial proteins are at best partially co-purifying contaminants of raft preparations.


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