| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
Papers In Press, published online ahead of print March 14, 2007
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Biochemistry, Weill Cornell Medical College, New York, NY 10021
Corresponding Author: akm2003{at}med.cornell.edu
Glycosylphosphatidylinositol (GPI) anchoring of cell surface proteins is the most complex and metabolically expensive of the lipid post-translational modifications described to date. The GPI anchor is synthesized via a membrane-bound multi-step pathway in the endoplasmic reticulum requiring more than 20 gene products. The pathway is initiated on the cytoplasmic side of the ER and completed in the ER lumen, necessitating flipping of a glycolipid intermediate across the membrane. The completed GPI anchor is attached to proteins that have been translocated across the ER membrane and which display a GPI signal anchor sequence at their C-terminus. GPI-proteins transit the secretory pathway to the cell surface; in yeast many become covalently attached to the cell wall. Genes encoding proteins involved in all but one of the predicted steps in the assembly of the GPI precursor glycolipid and its transfer to protein in mammals and yeast have now been identified. Most of these genes encode polytopic membrane proteins, some of which are organized in complexes. The steps in GPI assembly, and the enzymes that carry them out, are highly conserved. GPI biosynthesis is essential for viability in yeast and for embryonic development in mammals. In this review we describe the biosynthesis of mammalian and yeast GPIs, their transfer to protein, and their subsequent processing.
Revised on March 12, 2007
Accepted on March 13, 2007
GPI anchoring of protein in yeast and mammalian cells or: How we learned to stop worrying and love glycophospholipids
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Driouich and T. I. Baskin Intercourse between cell wall and cytoplasm exemplified by arabinogalactan proteins and cortical microtubules Am. J. Botany, December 1, 2008; 95(12): 1491 - 1497. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kinoshita, M. Fujita, and Y. Maeda Biosynthesis, Remodelling and Functions of Mammalian GPI-anchored Proteins: Recent Progress J. Biochem., September 1, 2008; 144(3): 287 - 294. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Signorell, M. Rauch, J. Jelk, M. A. J. Ferguson, and P. Butikofer Phosphatidylethanolamine in Trypanosoma brucei Is Organized in Two Separate Pools and Is Synthesized Exclusively by the Kennedy Pathway J. Biol. Chem., August 29, 2008; 283(35): 23636 - 23644. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Grabinska, S. K. Ghosh, Z. Guan, J. Cui, C. R. H. Raetz, P. W. Robbins, and J. Samuelson Dolichyl-Phosphate-Glucose Is Used To Make O-Glycans on Glycoproteins of Trichomonas vaginalis Eukaryot. Cell, August 1, 2008; 7(8): 1344 - 1351. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ashok and R. S. Hegde Retrotranslocation of Prion Proteins from the Endoplasmic Reticulum by Preventing GPI Signal Transamidation Mol. Biol. Cell, August 1, 2008; 19(8): 3463 - 3476. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Signorell, J. Jelk, M. Rauch, and P. Butikofer Phosphatidylethanolamine Is the Precursor of the Ethanolamine Phosphoglycerol Moiety Bound to Eukaryotic Elongation Factor 1A J. Biol. Chem., July 18, 2008; 283(29): 20320 - 20329. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Wilson, Q. Roebuck, and S. High Ribophorin I regulates substrate delivery to the oligosaccharyltransferase core PNAS, July 15, 2008; 105(28): 9534 - 9539. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-L. Chen, S. Kauffman, and T. B. Reynolds Candida albicans Uses Multiple Mechanisms To Acquire the Essential Metabolite Inositol during Infection Infect. Immun., June 1, 2008; 76(6): 2793 - 2801. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kajiwara, R. Watanabe, H. Pichler, K. Ihara, S. Murakami, H. Riezman, and K. Funato Yeast ARV1 Is Required for Efficient Delivery of an Early GPI Intermediate to the First Mannosyltransferase during GPI Assembly and Controls Lipid Flow from the Endoplasmic Reticulum Mol. Biol. Cell, May 1, 2008; 19(5): 2069 - 2082. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Umemura, M. Fujita, T. Yoko-o, A. Fukamizu, and Y. Jigami Saccharomyces cerevisiae CWH43 Is Involved in the Remodeling of the Lipid Moiety of GPI Anchors to Ceramides Mol. Biol. Cell, November 1, 2007; 18(11): 4304 - 4316. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
