Contributions of quantitative proteomics to understanding membrane microdomains
- Centre for High-Throughput Biology and Department of Biochemistry and Molecular Biology, 2125 East Mall, University of British Columbia, Vancouver, BC, Canada, V6T 1Z4
- 1To whom correspondence should be addressed. e-mail: ljfoster{at}interchange.ubc.ca
Abstract
Membrane microdomains, e.g., lipid rafts and caveolae, are crucial cell surface organelles responsible for many cellular signaling and communication events, which makes the characterization of their proteomes both interesting and valuable. They are large cellular complexes comprised of specific proteins and lipids, yet they are simple enough in composition to be amenable to modern LC/MS/MS methods for proteomics. However, the proteomic characterization of membrane microdomains by traditional qualitative mass spectrometry is insufficient for distinguishing true components of the microdomains from copurifying contaminants or for evaluating dynamic changes in the proteome compositions. In this review, we discuss the contributions quantitative proteomics has made to our understanding of the biology of membrane microdomains.
Footnotes
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- Abbreviations:
- BCR
- B-cell receptor
- 2DGE
- two-dimensional gel electrophoresis
- DRM
- detergent-resistant membrane
- ICAT
- Isotope-Coded Affinity Tags
- MβCD
- methyl-β-cyclodextrin
- PDGF
- platelet-derived growth factor
- SILAC
- Stable Isotope Labeling of Amino acids in Cell culture
- TCR
- T-cell receptor
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Operating funds for lipid raft research in the Cell Biology Proteomics (CBP) group come from a Canadian Institutes of Health Research Operating Grant (MOP-77688) to L.J.F. Infrastructure in the CBP, particularly the mass spectrometry facility, is supported by the Canada Foundation for Innovation, the British Columbia (BC) Knowledge Development Fund, and the Michael Smith Foundation through the BC Proteomics Network (BCPN). L.J.F. is the Canada Research Chair in Organelle Proteomics and a Michael Smith Foundation Scholar.
- Received June 9, 2009.
- Revision received June 30, 2009.
- Copyright © 2009 by the American Society for Biochemistry and Molecular Biology, Inc.
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