Perilipin-2-null mice are protected against diet-induced obesity, adipose inflammation, and fatty liver disease.

The cytoplasmic lipid droplet (CLD) protein perilipin-2 (Plin2) is expressed in multiple nonadipose tissues, where it is thought to play a role in regulating their lipid storage properties. However, the extent to which Plin2 functions in nutrient utilization and metabolism, or how it influences the consequences of over-feeding, remains unclear. In this study, we demonstrate that the absence of Plin2 prevents high-fat diet(HFD)-induced obesity in male and female mice. This response is associated with increased formation of subcutaneous beige adipocyte cells with uncoupling protein 1 expression, and amelioration of inflammatory foci formation in white adipose tissue and steatosis in the liver. Experiments demonstrate that Plin2 loss results in reduced energy intake and increased physical activity in response to HFD feeding. Our study provides the first evidence that Plin2 contributes to HFD-induced obesity by modulating food intake, and that its absence prevents obesity-associated adipose tissue inflammatory foci and liver steatosis.

Obesity, resulting from increased calorie consumption and decreased energy expenditure, remains a major public health concern for children and adults in the United States ( 1 ), through its strong etiological links to several metabolic complications, including metabolic syndrome, 22-24°C on a 12:12 h light-dark cycle with free access to food and water for the study's duration. All mice were fed standard mouse chow ad libitum from weaning to 8 weeks of age, at which time they were placed on isocaloric diets containing 60% fat calories (D12492) or 10% fat calories (D12450B) from Research Diets, Inc. Body weights were measured at weekly cage changes. Upon completion of the feeding experiments, determination of the body composition of each animal was performed by quantitative magnetic resonance EchoMRI-900 whole-body composition analyzer (Echo Medical Systems; Houston, TX), and blood samples were taken for serum analysis. For fasting-feeding experiments, male mice were fasted for 24 h then refed with the 60% fat calorie diet for 16 h. At the termination of each experiment, mice were injected with sodium pentobarbital to induce anesthesia and euthanized by exsanguination. After euthanization, organs and tissues were removed from each animal, weighed, and processed for histological, immunohistochemical, and biochemical analyses. The University of Colorado School of Medicine Institutional Animal Care and Use Committee approved all procedures.

Metabolic monitoring
Three separate cohorts of mice were placed in a metabolic monitoring system that provided measurements of energy balance (intake and expenditure), the respiratory exchange ratio (RER), and activity levels (Columbus, OH; 8M Oxymax) ( 16 ). Mice were individually housed in metabolic chambers after 9 weeks of HF feeding. Metabolic measurements were made following a 3 day lead-in to allow the mice to adjust to the chambers.

Histology and immunohistochemistry
Freshly dissected organ and tissue sections were processed for hematoxylin-and-eosin (H and E) staining and immunofl uorescence microscopy as described previously ( 17 ). Immunoreactivity was visualized using secondary antibodies conjugated with Alexafl uor 488 or Alexafl uor 594 at dilutions of 1:500 and 1:250, respectively. Nuclei were stained with (4 ′ ,6-diamidino-2phenylindole (Sigma Chemical Co.). Immunofl uorescence images were captured on a Nikon Diaphot fl uorescence microscope and digitally deconvolved using the No Neighbors algorithm (Slidebook; Denver, CO) as described previously ( 17 ). Histologic images were captured on an Olympus BX51 microscope equipped with a four megapixel Macrofi re digital camera (Optronics; Goleta, CA) using the PictureFrame Application 2.3 (Optronics). All images were cropped and assembled using Photoshop CS2 (Adobe Systems, Inc.; Mountain View, CA).

RNA extraction and transcript quantitation
Total RNA was extracted and its purity and integrity quantifi ed as described previously ( 15 ). PLIN family transcript copy numbers were determined by quantitative real-time PCR (qRT-PCR) analysis by the Quantitative Genomics Core Laboratory at the University of Texas Health Sciences Center using a multiplexing strategy, with validated primer-probe sets and 18S rRNA as a standard ( 15 ). The primers and probes used for qRT-PCR analyses are listed in Table 1 in the supplementary data. Three tissue replicates at each time point were analyzed.

Protein extraction and analyses
Liver samples were homogenized on ice with glass-on-glass grinding tubes in homogenization buffer (20 mM Tris-HCl, pH 7.4; 150 mM NaCl; 1% NP-40; 20 mM NaF; 2 mM EDTA, pH 8.0; 2.5 mM NaPP; 20 mM ␤ -glycerophosphate; 1% glycerol; ddH 2 O) plus protease and phosphatase inhibitors (aprotinin, leupeptin, pepstatin, AEBSF, PIC1, PIC2). Protein concentration of body fat accumulation ( 12,13 ). To explore the functional importance of Plin2 in obesity and other aspects of lipid metabolism, we generated a new conditional line of mice in which the Plin2 locus was disrupted by targeted removal of exon 5. In this paper, we document that mice lacking Plin2 are resistant to HFD-induced obesity. Our studies further show that Plin2 loss prevents HFD-induced obesity largely by limiting energy intake and altering locomotor activity.

Materials
Chemicals used were purchased from Sigma Chemical Co. (St. Louis, MO). Antibodies to N-and C-terminal regions of Plin2 and to Plin3 were raised in rabbits as described ( 15 ). Guinea pig antibodies to mouse Plin1, Plin4, and Plin5 were purchased from Fitzgerald (North Acton, MA). Antibodies to mouse F4/80 were purchased from Serotec (Raleigh, NC). HRP-conjugated and Alexfl uor-conjugated secondary antibodies were purchased from Life Technologies (Grand Island, NY). HF (60 kcal%; D12492) and low-fat (10 kcal%, D12045B) diets were purchased from Research Diets, Inc. (New Brunswick, NJ).

Generation of Plin2( ⌬ 5) mice
Mice lacking exon 5 of the Plin2 locus were generated using a targeting strategy that deleted 1.8 kb of the genomic sequence containing the murine Plin2 exon 5, resulting in out-of-frame splicing between exons 4 and 6 ( Fig. 1 ). A Flip recombinase target site (FRT) -neomycin-FRT-lox P cassette and a distal lox P site were inserted into introns 4 and 5, respectively, of a mouse genomic DNA encompassing Plin2 gene regions surrounding exon 5 that was cloned from a 129Sv/Pas miniBAC library (genOway; Lyon, France) and that is isogenic to the ES cell line used for homologous recombination. Short-and long-homology arms were generated using the following sets of primer pair sequences: Short-homology arm, forward, CGTGGGTAGCTTTATTTCCT-GAGTGACG, and reverse, CATAGGTATTGGCAACCGCAACTGC, amplify a 345 bp segment in intron 3 and exon 4 of Plin2. Longhomology arm, forward, TGGTGCATAGTACCTGCCTTCAAATGG, and reverse, TGGTGCATAGTACCTGCCTTCAAATGG, amplify a 549 bp segment in intron 7 of murine Plin2. 129/SvPas ES cells, containing an agouti coat color marker, were transfected with the linearized targeting vector and selected in G418-containing media. Homologous recombinations were identifi ed by PCR and Southern blotting. Targeted ES clones were injected into C57BL/6 blastocysts. Recombinant animals, identifi ed by coat color and PCR genotyping, were crossed with C57BL/6-expressing Cre-recombinase-expressing mice (CMV-Cre, genOway), and offspring were genotyped by PCR to identify those that were heterozygous for the knockout genotype. The knockout genotype of PCR-positive animals was confi rmed by Southern blot analysis. Heterozygous knockout animals were backcrossed to C57BL/6 mice for 15 generations to generate congenic C57Bl/6-Plin2( ⌬ 5) mice. Wild-type (WT) and Plin2( ⌬ 5) mice were genotyped by PCR using the following mixture of primer sequences that target exon 5 and adjacent intronic regions of the mouse Plin2 locus: 5 ′ -AGC AAC CTG ATG GAG ACA CTC AG -3 ′ (forward); 5 ′ -CAC TGT TCA TGA ACT GCA CCA TC -3 ′ (reverse 1); 5 ′ -CCG AGA GCA GAG CTT GGT AGA -3 ′ (reverse 2).

Animal housing and dietary treatments
Mice were housed in the University of Colorado School of Medicine's Center for Comparative Medicine at ambient temperatures of

Statistical analysis
Data were analyzed by Students t -test. Statistical signifi cance was assumed when P р 0.05.

Targeted disruption of exon 5 in the mouse Plin2 locus leads to loss of Plin2 expression
We generated Plin2( ⌬ 5) mice by crossing transgenic mice harboring lox P sites fl anking exon 5 of the Plin2 was measured using the DC Protein Assay Kit II (Bio-Rad Laboratories; Hercules, CA). Fifty microgram amounts of total protein were separated on 7.5% SDS-polyacrylamide gels, transferred to a 0.2 m nitrocellulose membrane, and probed with the 1/1,000 dilutions of rabbit antibodies to the N-and C-terminal regions of Plin2 ( 17 ). Corresponding HRP-conjugated secondary antibodies were used at 1/5,000 dilution. Bands were detected using SuperSignal West Pico chemiluminescent substrate (Thermo Fisher Scientific, Inc.; Rockford, IL), and band intensities were quantifi ed by densitometry using the ChemiDoc system (Bio-Rad Laboratories). Values are means ± SD normalized to 18S RNA. C: Exon 5 excision does not generate alternate splice forms of Plin2. The relative levels of exon 1-2, exon 4-5, and exon 8 products in fasted and refed livers of WT and Plin2( ⌬ 5) mice were quantifi ed by qRT-PCR and are shown as means ± SD normalized to 18S RNA. D: Plin2 is not detectable in livers of Plin2( ⌬ 5) mice. Representative immunoblots of liver extracts (50 g) from fasted and refed WT and Plin2( ⌬ 5) mice probed with antibodies to N-terminal (NT)-and C-terminal (CT)-specifi c sequences of mouse Plin2 ( 15 ). Bands corresponding to fulllength Plin2 in liver extracts from WT mice and the relative positions of selected molecular weight standards are indicated with arrows. Statistically signifi cant differences are indicated by asterisks locus with C57BL/6 mice globally expressing Crerecombinase, including in the germ line ( Fig. 1A ). Subsequently, the Cre gene was removed by mating, yielding mice expressing a mutated Plin2 locus under control of the CMV promoter in the absence of Cre expression. Mutant Plin2( ⌬ 5) mice on a mixed C57BL/6-129 background were then backcrossed with C57BL/6 males for over 15 generations. Congenic C57BL/6 mice lacking the Plin2 gene were identifi ed by PCR analysis (see supplementary Fig. I ). Primer sequences targeted to exon 5 and adjacent intronic regions (see Methods) amplifi ed a 387 bp band in WT mice and a 300 bp band in Plin2( ⌬ 5) mice. Excision of exon 5 was predicted to produce a truncated transcript in which exons 4 and 6 are fused, generating a frame-shift mutation and a premature stop codon in exon 7 that we hypothesized would result in degradation of the Plin2 mRNA ( Fig. 1A ).
We investigated whether exon 5 deletion disrupts Plin2 expression by determining Plin2 transcript and protein levels in livers of WT and Plin2( ⌬ 5) mice that had been fasted and then refed with a 60% fat diet. A fasting-andrefeeding protocol has previously been demonstrated to signifi cantly increase hepatic Plin2 mRNA and protein expression ( 18 ). Studies using qRT-PCR revealed that Plin2 transcript levels, in livers of WT mice, were at least 30-fold greater than those of other PLIN family members ( Fig. 1B ), indicating that Plin2 is the most highly expressed PLIN family member in livers from mice. To fully characterize how exon 5 removal affects Plin2 transcript properties, we probed hepatic RNA with primers that included the boundaries of exons 1-2 and exons 4-5, and exon 8. We did not detect exon 4-5 or exon 8 Plin2 transcripts in hepatic RNA from Plin2( ⌬ 5) mice ( Fig. 1C ), confi rming that loss of exon 5 disrupts transcription of downstream exons ( Fig. 1A ). We were able to detect low levels of Plin2 mRNA at the exon 1-2 junction. However, levels of this product were less than 10% of that obtained from hepatic RNA of WT mice, indicating that loss of exon 5 also impairs upstream exon transcription and/or processing. To determine whether mice harboring the Plin2( ⌬ 5) mutation produce a protein product, we probed liver extracts from fasted and refed WT and Plin2( ⌬ 5) mice with antibodies specifi c to N-terminal and C-terminal sequences of mouse Plin2 ( 15 ). Figure 1D demonstrates that both antibodies detected a band in extracts of WT livers corresponding in size to that of full-length Plin2. However, neither antibody detected bands in liver extracts from Plin2( ⌬ 5) mice, which is consistent with the absence of Plin2 protein expression in these animals. Additionally, the relative mRNA levels of other PLIN family members in Plin2( ⌬ 5) livers were comparable to thosefound in WT livers ( Fig. 1B ), suggesting that disruption of the Plin2 locus does not induce compensatory changes in expression of other PLIN proteins in livers of fasted and refed mice.

Plin2-defi cient mice are resistant to obesity induced by an HF diet
Having established that Plin2( ⌬ 5) mice lack detectable amounts of Plin2, we next investigated the effects of Plin2 loss on body fat accumulation in response to HF diet feeding. Like other Plin2-defi cient strains ( 19 ), we found that Plin2( ⌬ 5) females had diffi culty raising their fi rst litters. We are currently investigating the nature of this defect. However, Plin2( ⌬ 5) mice derived from subsequent pregnancies had normal viability and growth rates, and by 8 weeks of age, when HF feeding was started, their body weights were similar to those of WT mice ( Fig. 2A ).
We examined the effects of a high caloric diet in cohorts of 8 week-old male WT and Plin2( ⌬ 5) mice utilizing a lowfat (LF) diet (10% fat calories) or a HF diet (60% fat calories) for 8 to 12 weeks ( Fig. 2 ). We found that providing mice ad libitum with a LF diet increased the average weights of both WT and Plin2( ⌬ 5) mice by about 20% after 8 weeks ( Fig. 2B ). These weights were not signifi cantly different from each other ( P = 0.43), thus demonstrating that Plin2 loss does not affect weight gains on a LF diet. However, on a HF diet, Plin2( ⌬ 5) mice gained signifi cantly less weight than did WT mice over an 8-12 week feeding period ( Fig. 2A, B ). The body weights of WT mice on the HF diet increased by 16 g after 8 weeks and 22 g after 12 weeks ( Fig. 2A , inset), whereas the weights of Plin2( ⌬ 5) mice on the HF diet increased by only 8 g after 8 weeks and only 14 g after 12 weeks. Beginning on week 5 of HF feeding, the body weights of WT mice were signifi cantly ( P < 0.05) greater than those of Plin2( ⌬ 5) mice. These results suggest that Plin2 loss selectively reduces weight gains associated with HF feeding in male mice. We performed similar studies in female mice and found that although females weighed much less than males, the average body weights and the percent weight gain of Plin2( ⌬ 5) females fed the HF diet were also signifi cantly less ( P < 0.01) than those of HF-fed WT females ( Fig. 2C ).

HF diet-induced increases in fat accumulation are prevented by Plin2 loss
To determine whether the decreased weight gain observed in HF-fed Plin2( ⌬ 5) mice was associated with selective reduction of fat mass, we quantifi ed the amounts of lean and fat mass in WT and Plin2( ⌬ 5) mice fed the HF diet for 9 weeks ( Fig. 3A ). Whereas fat mass averages for Plin2( ⌬ 5) mice were 60% ( P < 0.003) less than those of WT controls, Plin2 loss did not appear to affect lean mass quantities ( Fig. 3A ). For WT and Plin2( ⌬ 5) males on the HF diet, lean mass values after 9 weeks on the diet were comparable to their starting lean masses ( Fig. 3B ). In contrast, fat mass values for WT males increased by nearly 275%, whereas fat mass values for Plin2( ⌬ 5) mice increased by only about 40% ( Fig. 3B ).
We also investigated whether the failure to detect effects on HF diet-induced weight gains and body fat mass in other models of Plin2 defi ciency ( 12,13 ) was related to differences in the genetic models used for disrupting Plin2 expression, or to the length of the HF feeding periods. We compared the relative weight gains of 8 week-old male mice fed LF or HF diets for 8 weeks ( Fig. 3C ). Weight gain of Plin2( ⌬ 2,3) mice had previously been compared with WT mice on a 45% fat diet for only 4 weeks ( 12 ). The weights of WT and Plin2( ⌬ 2,3) mice both increased by approximately 20% over their starting values on the LF diet; and at 9 weeks, their weights were not statistically different. In contrast, on the HF diet, the relative weight gains of Plin2( ⌬ 2,3) mice after 9 weeks (30%) were signifi cantly droplets ( Fig. 5B , panels a and b). Epididymal adipose tissue from Plin2( ⌬ 5) mice was also composed of adipocytes with unilocular fat droplets ( Fig. 5B , panel c). However, Plin2( ⌬ 5) subcutaneous adipose depots contained extensive areas in which adipocytes possessed multilocular lipid droplets ( Fig. 5B , panel d). We immunostained subcutaneous adipose tissue with antibodies to the adipose lipid droplet protein, Plin1, to further establish this observation. Figure 5C demonstrates Plin1 (green staining) localizing to multiple small lipid droplets in subcutaneous adipocytes from Plin2( ⌬ 5) mice. To confi rm the effects of Plin2 loss on adipocyte size, we determined the average size of adipocytes in multiple sections from epididymal and subcutaneous adipose tissue from WT and Plin2( ⌬ 5) mice fed the HF diet for 12 weeks. The average diameters of epididymal and subcutaneous adipocytes in HF-fed WT mice were 48 m and 46 m, respectively. Loss of Plin2 was associated with a 20% decrease in the relative size of epididymal adipocytes ( P < 0.01) and a nearly 40% decrease in the relative size of subcutaneous adipocytes ( P < 0.001) ( Fig. 5D ).
Plin2 is expressed by adipocytes and localizes to small CLDs during their early differentiation, where it is proposed to contribute to the management of triglyceride (TG ) storage during adipose differentiation ( 20,21 ). Consistent with this general notion, the presence of adipocytes with multilocular droplets within subcutaneous adipose tissue of Plin2( ⌬ 5) mice indicates that Plin2 loss may infl uence development of adipocytes with properties of brown adipocytes, such as UCP1 expression ( 22 ). In epididymal ( P < 0.01) less than those of WT mice (60%) ( Fig. 3C ). Similar to HF-fed Plin2( ⌬ 5) mice, Plin2( ⌬ 2,3) mice had signifi cantly lower fat mass values ( P < 0.01) than their WT counterparts ( Fig. 3D ) after 9 weeks of feeding, and signifi cantly greater lean mass values ( P < 0.01). We also found that female Plin2( ⌬ 5) mice were similarly protected against the dramatic increase in fat mass as compared with WT mice ( Fig. 3E ). Thus, in mice, the effects of Plin2 loss on HF diet gains in adiposity appear to be independent of both gender and genetic models.
Consistent with decreased amounts of body fat, we found that overnight fasting serum leptin levels in HF-fed Plin2( ⌬ 5) mice were about a third of those found in WT mice ( P < 0.04) ( Table 1 ). We also found that fasting levels of serum insulin were lower in Plin2( ⌬ 5) mice compared with WT mice, although the difference did not reach statistical signifi cance ( P = 0.07) ( Table 2 ).

Plin2-null mice on a HF diet have reduced food intake and increased activity levels
To determine whether the resistance of Plin2( ⌬ 5) mice to HF diet-induced weight gains was associated with alterations in energy consumption and/or expenditure, we compared food intake, energy expenditure, and activity levels of male WT and Plin2( ⌬ 5) mice. The weekly food consumption of Plin2( ⌬ 5) mice was less than that of WT mice, reaching signifi cance around week 4 of HF feeding ( Fig. 4A ). In agreement with food consumption measurements, we found that energy intake values, as determined by indirect calorimetry, of male Plin2( ⌬ 5) mice fed the HF diet for 8 weeks were signifi cantly less ( P < 0.01) than those of WT mice ( Fig. 4B ), whereas their weight-adjusted energy expenditures were similar to those of WT males ( Fig.  4B ). The consequence of these differences was that after 8 weeks of HF feeding, WT mice had a positive energy imbalance of about 8 Kcal/day, whereas Plin2( ⌬ 5) mice had a signifi cantly lower ( P < 0.02) positive energy imbalance of only about 2 Kcal/day. We did not detect differences in the type of fuel utilized for energy by HF-fed Plin2( ⌬ 5) and WT mice as determined by RER, nor did we fi nd differences in the amount of fat in their feces (data not shown). Interestingly, the ambulatory activity of Plin2( ⌬ 5) mice after 8 weeks of HF feeding was signifi cantly greater than that of WT mice (33%, P < 0.01, data not shown). Collectively, these data indicate that diminished energy intake and possibly increased activity are the primary contributors to the resistance of Plin2( ⌬ 5) mice to HF diet-induced weight gain.

Plin2 absence reduces adipocyte cell size and infl ammatory cell foci and results in appearance of beige adipocytes
Consistent with a decrease in total body fat on a HF diet, we found that after 12 weeks of HF feeding, the epididymal and subcutaneous adipose depot weights of Plin2( ⌬ 5) mice were signifi cantly less than those of WT mice ( Fig. 5A ). Histological analysis revealed that adipocytes in visceral and subcutaneous adipose depots of WT mice were large and contained mainly unilocular fat  hallmark of obesity-associated adipose tissue infl ammation is the appearance of crown-like structures (CLSs), which are composed of macrophages localized around, and scavenging, the lipid remnants of dead adipocytes ( 24 ). We quantifi ed CLSs in visceral and subcutaneous adipose tissue of WT and Plin2( ⌬ 5) mice fed the HF diet for 12 weeks ( Fig. 6 ). Signifi cant numbers of CLSs were evident in epididymal (visceral) adipose tissue of WT mice ( Fig. 6, panels A and D). We verifi ed that these CLSs were composed of macrophages by F4/80 immunohistochemical staining ( Fig. 6B ). In agreement with previous studies ( 24 ), we found that CLS macrophages were enriched in Plin2-coated lipid droplets ( Fig. 6C ). In contrast, we did not detect signifi cant numbers of CLSs in epididymal adipose tissue of HF-fed Plin2( ⌬ 5) mice, or in subcutaneous adipose tissue of WT or Plin2( ⌬ 5) mice ( Fig. 6D ). The reduction in CLSs is consistent with and perhaps explained by the reduced size of epididymal adipocytes observed in HF-fed Plin2( ⌬ 5) mice.

Plin2 loss prevents obesity-associated fatty liver disease
Nonalcoholic fatty liver disease is a common pathological feature of obesity in humans and in rodent models ( 25,26 ). Because Plin2 defi ciency has been reported to reduce lipid accumulation in livers of mice following 4 week exposure to a 45% fat diet ( 12, 13 ), we investigated whether our line of Plin2-null mice also prevented hepatic pathology associated with obesity in a prolonged feeding model. H and E staining of liver sections from WT and Plin2( ⌬ 5) mice fed the HF diet for 12 weeks showed signifi cant steatosis in livers of WT but not Plin2( ⌬ 5) mice ( Fig. 7A ). In livers of WT mice, we observed a microvesicular pattern (vesicle diameter <10 m) of steatosis in hepatocytes adjacent to the central vein (zone 3) and in the midlobular region (zone 2) (small arrow, Fig. 7A ). In addition, we observed a macrovesicular pattern (vesicle diameter >10 m) of steatosis in the peri-portal region (zone 1) (large arrow, Fig. 7A ). However, this macrovesicular pattern was attenuated and not present in all hepatocytes. Consistent with this histological evidence, we found that total TG in Plin2( ⌬ 5) livers was only about 10% of that found in WT livers ( Fig. 7B ) ( P < 0.001), and that serum TG and FA levels were signifi cantly lower ( P < 0.008) in Plin2( ⌬ 5) mice compared with WT mice ( Table 1 ). The effects of a HF diet on liver pathology in WT and Plin2( ⌬ 5) mice were categorized using the Kleiner/Brunt system as adapted to rodents ( 26 ) ( Table 2 ). The results show that after 12 weeks on the HF diet, in addition to steatosis, WT mice exhibited a limited degree of additional liver pathology, predominantly microgranulomas, whereas evidence of infl ammation and fatty liver disease was not detected in Plin2( ⌬ 5) mice.
Plin2 defi ciency in hepatocytes has been suggested to increase activation of peroxisome proliferator-activated receptor ␣ (PPAR ␣ ) ( 27 ). We quantifi ed transcript levels of PPAR ␣ in livers of WT and Plin2( ⌬ 5) mice fed HF or LF diets for 12 weeks ( Fig. 7C ). In animals on the LF diet, we found that hepatic PPAR ␣ transcript levels in Plin2( ⌬ 5) mice were nearly 50% higher than those of WT mice adipose tissue of 12 week HF-fed animals, we detected no difference in UCP1 expression between WT and Plin2( ⌬ 5) mice ( Fig. 5E ). However, in subcutaneous adipose tissue, UCP1 transcript levels in Plin2( ⌬ 5) mice were signifi cantly elevated over WT levels ( P < 0.005). The mRNA of PGC-1 ␣ , an important coactivator involved in mitochondrial biogenesis ( 23 ), also tended to be greater in epididymal adipose tissue of HF-fed Plin2( ⌬ 5) mice compared with WT mice, and greater than the levels found in subcutaneous adipose tissue of WT mice ( Fig. 5E ). However, the differences did not reach statistical signifi cance ( P = 0.08). We also found that Plin2 loss was associated with significant increase in the relative expression levels of UCP1 (WT = 1.00 ± 0.22, N = 5; Plin2( ⌬ 5) = 54.23 ± 20.05, N = 5, P = 0.029), and PGC-1 ␣ (WT = 1.00 ± 0.06, N = 5; Plin2( ⌬ 5) = 2.55 ± 0.26, N = 5, P = 0.004) in subcutaneous adipose tissue of chow-fed mice. In summary, these fi ndings suggest that subcutaneous adipose tissue in our line of Plin2-null mice exhibits properties of brown-like, or beige, adipocytes; and that when on a HF diet, adipocyte size in epididymal and subcutaneous adipose tissue is reduced by the absence of Plin2.
Obesity-associated infl ammation has been linked to the development of metabolic complications. A histological of WT mice ( Fig. 7C ). We did not detect differences in PPAR ␥ transcript levels in livers of WT and Plin2( ⌬ 5) mice fed the LF diet ( Fig. 7C ).
In addition to Plin2, Plin1 and Plin3 have been shown to coat hepatic CLDs in humans and mice under certain pathological conditions ( 25,26 ), and there is evidence that Plin3 and -5 are capable of partially compensating for loss of Plin2 in stabilizing CLD accumulation in some cell types ( 31 ). To assess the possible roles of other PLIN family members in the hepatic responses to a chronic HF diet in WT and Plin2( ⌬ 5) mice, and to determine if loss of Plin2 is associated with compensatory changes in other PLIN family members, we investigated the entire PLIN ( P < 0.005). Consistent with known stimulating effects of elevated FAs on PPAR ␣ expression ( 28 ), we found that there was a signifi cant increase in PPAR ␣ transcript levels in 12 week HF-fed WT mice compared with mice on the LF diet ( P < 0.007). However, PPAR ␣ transcript levels in 12 week HF-fed Plin2( ⌬ 5) mice did not differ from those of LF-fed littermates or HF-fed WT mice. Elevated PPAR ␥ expression is also thought to be a general property of fatty livers ( 29 ), and to possibly contribute to hepatic TG regulation ( 30 ). Consistent with the observation that Plin2( ⌬ 5) mice on a HFD are protected against development of fatty liver, we found that hepatic PPAR ␥ transcript levels of HF-fed Plin2( ⌬ 5) mice were signifi cantly ( P < 0.01) less than those Plin1 or Plin4 immunoreactivity in extracts of livers from WT or Plin2( ⌬ 5) mice (data not shown). Liver extracts from WT and Plin2( ⌬ 5) mice on the HF diet had similar levels of Plin3, whereas Plin5 immunoreactivity was detected only in liver extracts of Plin2( ⌬ 5) mice. Immunohistochemical staining of liver sections from 12 week HF-fed WT and Plin2( ⌬ 5) mice demonstrated that hepatocyte CLDs in WT mice were selectively coated with Plin2 ( Fig. 7F , panel a). In contrast, the few hepatocyte CLDs present in Plin2( ⌬ 5) mice were selectively coated with Plin5 ( Fig. 7F , panel j), whereas Plin3-coated CLDs were found in stellate cells located in the sinusoids ( Fig. 7F ,  panel f). Interestingly, increased Plin5 levels are typically expressed in cells that have enhanced capacity for lipid oxidation ( 35,36 ), and its expression is increased in Plin2( ⌬ 5) livers. In summary, absence of Plin2 expression in liver protects against development of NAFLD in response to a HF diet and is associated with increased expression of Plin5. family member expression profi le. Figure 7D shows the relative transcript levels of PLIN family members in extracts of livers of these mice after HF or LF feeding for 12 weeks. We did not detect transcripts for Plin1 in liver extracts of WT or Plin2( ⌬ 5) mice on either diet. Transcripts for Plins 2-5 were detected in livers of HF-and LF-fed WT mice; however, Plin2 transcript levels were 40-70 times greater than those of the other PLIN family members ( Fig. 7D ). Consistent with the known effects of HF feeding on Plin2 expression ( 32 ), we found that transcript levels of Plin2 in liver extracts of HF-fed WT mice were approximately twice those found in extracts of LF-fed mice ( P < 0.01). However, transcript levels for Plins 3-5 were not affected by diet. Because the regulation of PLIN family member levels occurs by transcriptional and posttranscriptional mechanisms ( 33,34 ), we next investigated by immunoblot analysis whether Plin2 loss affects other PLIN family member protein levels in livers of mice fed the HF diet for 12 weeks ( Fig. 7E ). We did not detect signifi cant

DISCUSSION
Our data provide several new insights into the physiological functions of Plin2 in mice. Utilizing a novel Plin2null mouse model [Plin2( ⌬ 5)], we demonstrate for the fi rst time that Plin2 loss interferes with HF diet-induced obesity, and that the physiological basis of this effect appears, in part, to be related to reduced energy intake, and possibly to increased activity. Previous studies, using genetic or antisense models of Plin2 defi ciency, failed to characterize effects of Plin2 loss on diet-induced obesity ( 12,13 ). Our data suggest that this was not observed in these prior studies, in part due to insuffi cient time of exposure to HF diets, although in the case of the antisense model, it is possible that failure to detect HF diet-induced changes in body weight were also related to a more tissue restricted knockdown of Plin2. We found that differences in the weights of WT C57BL/6 mice and C57BL/6 mice lacking Plin2 were not statistically apparent until after about 4 weeks of HF diet feeding, which was the total length of time of HF diet exposure in the previous studies ( 12,13 ). Signifi cantly, we found that when exposed to HF diet feeding for 12 weeks, the Plin2( ⌬ 2,3) mouse model of Chang et al. ( 12 ) was also resistant to diet-induced obesity. Mammary glands of lactating female Plin2( ⌬ 2,3) mice have been shown to produce an N-terminal-truncated form of Plin2 ( 15 ). However, we did not detect Plin2 products in liver extracts of male Plin2( ⌬ 2,3) mice using N-or C-terminal-specifi c antibodies (data not shown), which is consistent with original observations that nonlactating Plin2( ⌬ 2,3) mice are defi cient in Plin2 ( 12 ). Thus, there appears to be general agreement in the responses of Plin2( ⌬ 2,3) and Plin2( ⌬ 5) models to prolonged HF diet feeding, demonstrating in both models that loss of Plin2 impairs obesity development.
Effects of Plin2 defi ciency on energy intake have not been identifi ed previously. Our data indicate that differences in weight gains between WT and Plin2-null mice occur after about 4 weeks of HF diet feeding and correlate with decreased food consumption by Plin2-null animals. These fi ndings suggest that decreases in energy intake related to loss of Plin2 may be an adaptive response to HF feeding. Reductions in food intake may also have been an important contributor in the observed reductions in serum TG and FAs in Plin2( ⌬ 5) mice. The mechanism(s) by which Plin2 infl uences energy intake and activity are unclear as yet, and may involve multiple organ systems, given Plin2's ubiquitous tissue distribution. To address the underlying mechanisms and role of specifi c tissues in mediating the effects of Plin2 ablation on food intake and energy metabolism in future studies, we will generate mouse lines in which Plin2 is ablated in specifi c tissues.
Plin2 defi ciency induced by treatment with Plin2 antisense oligonucleotides has been linked previously to decreased fat mass in HF-fed mice ( 13 ). However, the effects of Plin2 defi ciency on adipose content have not been consistent; for instance, in the Plin2( ⌬ 2,3) model, Plin2 loss did not appear to affect the content or properties of adipose tissue ( 12 ). However, in these studies, the authors did not examine subcutaneous adipose tissue in their line of Plin2-null mice. Our data demonstrate that compared with WT mice, both Plin2( ⌬ 2,3) and Plin2( ⌬ 5) mice exhibit a similar reduction in fat mass after 12 weeks of HF feeding. Thus, in three different models, Plin2 loss is associated with the curtailment of fat mass increases due to long-term HF feeding. Further, the correlation of Plin2 loss with the presence in subcutaneous adipose tissue of smaller adipocytes, multilocular CLDs, and elevated expression of UCP1 and PGC1 ␣ genes, suggests that Plin2 may play a role in regulating adipose metabolic properties, and possibly conversion of white to beige adipose tissue. Because we did not observe alterations in metabolic rate in our study, the effects of beige cells in subcutaneous adipose tissue on the physiology of Plin2 mice are unclear and will be explored in future studies. How Plin2 affects adipose properties remains to be defi ned. However, it is conceivable that Plin2 could act indirectly through effects on energy intake regulation and/or directly through regulation of adipose lipid packaging ( 21 ).
Macrophage infi ltration to form infl ammatory foci in adipose tissue is a key feature of obesity-related pathology in humans and rodents ( 24,37 ). Our data showing that the number these foci is dramatically decreased in epididymal adipose tissue of Plin2-null animals compared with WT controls provide the fi rst evidence implicating Plin2 in obesity-dependent adipose pathophysiology. Infl ammatory foci formation is known to positively correlate with adipocyte size ( 38 ). The observation that Plin2 loss results in signifi cant decreases in the size of adipocytes in both the epididymal and subcutaneous adipose tissue suggests that Plin2 expression may contribute to infl ammatory foci formation through effects on epididymal adipocyte size.
NAFLD is another common pathological feature of obesity. Plin2 defi ciency has been documented previously to reduce fatty liver associated with HF feeding ( 12,13 ). Our data extend this fi nding to show that Plin2 loss completely prevents the hepatosteatosis and other aspects of fatty liver disease that occur with 12 weeks of HF feeding in WT mice. Loss of hepatic Plin2 was further shown to be associated with dramatic upregulation of Plin5 levels in hepatocytes of HF-fed mice. Plin5 is most highly expressed in oxidative tissues such as skeletal muscle, and it was initially thought to promote fat oxidation ( 18,36 ). However, more-recent data suggest that Plin5 protects mitochondria against the increased fl ux of FAs to mitochondria that leads to oxidative stress ( 39,40 ). Thus, expression of Plin5 may act to maintain the physiology of the hepatocyte in response to increased traffi cking of FAs to mitochondria. Plin3 has been implicated as a possible regulator of lipid droplet accumulation in the absence of Plin2 ( 31 ). Although loss of Plin3 is associated with a mild reduction in hepatosteatosis in mice ( 41 ), our data showing that Plin3 selectively localizes to lipid droplets in stellate cells, but not hepatocytes, in livers of Plin2-null mice suggest that its role in hepatosteatosis may not be directly related to hepatocyte functions.
In summary, we document in two genetic mouse models that Plin2 loss attenuates diet-induced obesity, and further, we demonstrate that loss of Plin2 prevents obesityassociated adipose and hepatic pathological changes in mice. Although additional studies are needed to establish the physiological mechanisms underlying the effects of Plin2 on obesity, our data suggest that Plin2 plays an important role in energy homeostasis. We observed that normal Plin2 expression in the C57BL/6 line is critical to the obesity-prone phenotype that manifests with HF feeding. The loss of Plin2 yields an obesity-resistant phenotype characterized by adaptations in food intake and physical activity in response to this chronic dietary challenge. The gradual and integrative nature of this response may explain why Plin2 loss did not affect weight gains during shorter exposures to the HF diet ( 12,13 ) or why Plin2 loss does not affect body weights of mice in which leptin receptor signaling is disrupted ( 42 ). Understanding how Plin2 is involved in the homeostatic control of energy balance will provide new insights into the mechanisms by which nutrition overload is detected and how individuals adapt to, or fail to adapt to, dietary challenges.