Carboxy-terminal mutations of bile acid CoA:N-acyltransferase alter activity and substrate specificity

Bile acid CoA:amino acid N-acyltransferase (BAAT) is the terminal enzyme in the synthesis of bile salts from cholesterol and catalyzes the conjugation of taurine or glycine to bile acid CoA thioesters to form bile acid N-acylamidates. BAAT has a dual localization to the cytosol and peroxisomes, possibly due to an inefficient carboxy-terminal peroxisomal targeting signal (PTS), -serine-glutamine-leucine (-SQL). Mutational analysis was used to define the role of the carboxy terminus in peroxisomal localization and kinetic activity. Amidation activity of BAAT and BAAT lacking the final two amino acids (AAs) (BAAT-S) were similar, whereas the activity of BAAT with a canonical PTS sequence (BAAT-SKL) was increased >2.5-fold. Kinetic analysis of BAAT and BAAT-SKL showed that BAAT-SKL had a lower Km for taurine and glycine as well as a greater Vmax. There was no difference in the affinity for cholyl-CoA. In contrast to BAAT, BAAT-SKL forms bile acid N-acylamidates with β-alanine. BAAT-S immunoprecipitated when incubated with peroxisomal biogenesis factor 5 (Pex5) and rabbit anti-Pex5 antibodies; however, deleting the final 12 AAs prevented coimmunoprecipitation with Pex5, indicating the Pex5 interaction involves more than the -SQL sequence. These results indicate that even small changes in the carboxy terminus of BAAT can have significant effects on activity and substrate specificity.

(underlined) and the reverse primers contained a HindIII restriction site (underlined) to facilitate cloning into pKK233-2 for bacterial expression. To change the wild-type BAAT PTS-1 sequence (-SQL) to a more canonical sequence (-SKL), Gln417 was changed to a Lys by mutating the hBAAT sequence from CAA to AAA using the forward primer 5′-CAGACCATGGTCCAGTT-GACAGCTAC-3′ and the reverse primer 5′-CCCAAGCTTT-GGGCTTAGAGTTTACTGGTCACATCTGG-3′. The generated Lys codon is in bold. The final 12 AAs were removed from the C terminus of BAAT by using the forward primer 5′-CAGAC-CATGGTCCAGTTGACAGCTAC-3′ and the reverse primer 5′-CAAGCTTGTTAGAGAAATCTCTGGATCTCCTTCCAAG -3′ to introduce a termination codon at AA position 407 by mutating the nucleotide 1204 from C→A. The termination codon is in bold. The first exon of BAAT (147 AA) was deleted by amplifying the remaining codons using the forward primer 5′-CCATGG-GAGCTCTTTCTCCCTCCAG-3′ and the reverse primer 5′-CCC-AAGCTTTGGGCTTAGAGTTGACTGGTCACATCTGG-3′. The truncated BAAT sequence was subcloned into pKK233-2 for expression.

Bacterial expression and purification of hBAAT
To obtain active purified hBAAT protein, an overnight culture of Escherichia coli DH5 containing a pKK-hBAAT plasmid previously constructed by our laboratory (13) was diluted 1:100 in LB broth containing 100 g/ml ampicillin. This culture was grown to an OD 600 = 0.5. IPTG was added to a final concentration of 0.5 mM to induce expression of hBAAT, and the culture incubated an additional 2 h. The culture was centrifuged at 3,000 g for 15 min to pellet the bacteria; then the pellet was resuspended in bacterial lysis buffer [75 mM Tris (pH 8), 250 mM sucrose, 0.25 mM EDTA, 0.02 mg/ml lysozyme] and incubated for 20 min on ice. The lysate was centrifuged at 3,000 g at 4°C for 10 min, resuspended in TEA buffer [10 mM triethanolamine (pH 8), 1.5 mM DTT, 10% glycerol, protease inhibitors (Complete Mini; Roche Biosciences)], and sonicated on ice four times for 10 s each with 30 s in between pulses. The lysed bacteria were centrifuged at 100,000 g for 1 h, and the resulting cytosolic fraction was retained.
For further purification of expressed hBAAT, the bacterial cytosol was loaded onto a 17 × 1.5 cm DEAE-Sepharose Cl-6B column. The column was washed with 30 ml of TEA buffer, and hBAAT was eluted with a 0-200 mM NaCl gradient and collected in 3 ml fractions. Fractions containing hBAAT were identified by BAAT activity assays and immunoblotting. Fractions enriched in BAAT activity were pooled and used for characterization.

Immunoblot analysis
For immunoblot analysis, protein fractions (100 g) were resolved by SDS-PAGE and electrotransferred to nitrocellulose membranes. Membranes were blocked with 5% nonfat milk followed by incubation with a 1:5,000 dilution of rabbit anti-mouse BAAT antibody, described previously (14), or a commercially available goat anti-human Pex5 antibody (Santa Cruz). Membranes were then incubated with a 1:20,000 dilution of goat anti-rabbit IgG conjugated with horseradish peroxidase (for BAAT antibody) or a 1:20,000 dilution of donkey anti-goat IgG conjugated with horseradish peroxidase (for Pex5 antibody). Immunoconjugates were visualized using the Supersignal West Pico system (Pierce).

Bacterial expression and purification of human Pex5S
Cloned human Pex5S cDNA (AK225126) in a PCMV6-XL4 vector was purchased from Origene (Rockville, MD). For bacterial expression, Pex5 was amplified by PCR using the To date, the investigation of the AA residues affecting BAAT activity has been limited to residues considered as part of the active site located near the interior of the protein (3). The carboxy (C)-termini of N-acetyltransferases have been shown to be critical for activity, and the final three AAs of BAAT are thought to associate with peroxisomal biogenesis factor 5 (Pex5) and facilitate translocation to peroxisomes (5,6). Approximately 25-30% of BAAT in hepatocytes is localized to peroxisomes; the remainder is in the cytosol (7)(8)(9). The majority of proteins targeted to peroxisomes utilize a three AA carboxy-terminal peroxisomal targeting signal (PTS)-1 sequence that interacts with Pex5 (10,11). Typically, the PTS-1 sequence is -serine/alanine-arginine/lysine-leucine/methionine [S/A] [R/K][L/M] located at the C-terminus (12); however, BAAT contains a degenerate PTS-1 sequence, -serine-glutamine-leucine (-SQL) (13). Because approximately 25% of BAAT is localized in peroxisomes (7)(8)(9), it was hypothesized that the BAAT-SQL sequence was responsible for a lower level of binding to Pex5 and, thus, a limited targeting of BAAT to peroxisomes. Here, the changes in the activity and properties of BAAT conferred by specific mutations in the C-terminus of the protein are examined. Relatively minor changes in the sequence of BAAT at the C-terminus had significant effects on BAAT activity and substrate selectivity, indicating the importance of this region in the catalytic properties of the enzyme. A point mutation in the penultimate C-terminal residue of BAAT, resulting in formation of a canonical Pex5 binding sequence (BAAT-SKL), increased amidation activity, thioesterase activity, and resistance to thermal inactivation. The PTS sequence was not necessary for interacting with Pex5, as evaluated by immunoprecipitation; however, this sequence plays an important role in the recognition of AAs capable of conjugating the thiol-bile acid intermediate.

Construction of BAAT mutants
To construct C-terminal mutants, a pKK233-2-hBAAT construct (13) was utilized for PCR mutagenesis. To disrupt the PTS-1 sequence, the Gln417 residue was changed to a termination codon by converting nucleotide 1247 from a C→T using the forward primer 5′-CAGACCATGGTCCAGTTGACAGCTAC-3′ and reverse primer 5′-CCCAAGCTTGGGCTTAGAGTTAACTG-GTCACATCTGG-3′. The generated TAA termination codon is in bold. The forward primers contained an NcoI restriction site internal standards were taurodeoxycholic acid and chenodeoxycholic acid. Aliquots of the reactions containing internal standards were then injected into a reverse-phase column (RP-300 C 8 , 4.6 × 100 mm) by an autosampler. Solvent A was 10 mM ammonium acetate in 10% aqueous acetonitrile and solvent B was 10 mM ammonium acetate in 100% acetonitrile. The samples were run on a linear gradient from 100% solvent A to 100% solvent B to elute and introduced into the ESI interface of the API 3 triple quadrupole mass spectrometer (Sciex, Concord, Ontario, Canada) operating in the negative ion mode. The precursor ion/product ion transitions (m/z 407/343 for cholic acid; m/z 391/345 for chenodeoxycholic acid; m/z 514/124 for taurocholic acid; m/z 498/124 for taurodeoxycholic acid) were monitored to detect the formation of cholic acid and taurocholic acid.
To examine the reaction products of the different BAAT forms with nortaurine and homotaurine, the reaction described for the radioassay was used, but taurine was replaced by nortaurine or homotaurine. After incubation for 10 min, enzyme reactions were stopped by addition of 500 l of methanol, and aliquots of the supernatant infused into the mass spectrometer in 50% methanol-0.1% acetic acid, recording negative mass spectra between m/z 400 and 600.

hBAAT mutants
To examine the role of the BAAT-SQL and the adjacent sequence in Pex5 binding and regulation of enzymatic activity, several sequence variants were made to the carboxy terminal of BAAT. A truncated form of hBAAT was generated by removing the terminal Gln417 and Leu418 residues of the PTS sequence, resulting in a carboxy terminus ending in Ser, termed hBAAT-S ( Fig. 1). Conversely, the penultimate residue of wild-type BAAT, the Gln of the PTS sequence was changed to a Lys to generate BAAT with a canonical PTS sequence, hBAAT-SKL (Fig. 1). The 12 carboxy-terminal AAs (407-418) were also removed (hBAAT-12AA Trunc) because Brocard and Hartig (19) had reported that a dodecapeptide sequence was necessary for the targeting of certain proteins to peroxisomes instead of a three AA PTS-1 sequence. Additionally, an N-terminal PCMV6-XL4-Pex5 vector as a template with the forward primer 5′-CCCGGGCCATGGCAATGCGGAG-3′ and the reverse primer 5′-GTCGACTCACTGGGGCAGGCCAAAC-3′. The forward primer contained an XmaI restriction site (underlined) and the reverse primer contained a SalI restriction site (underlined) to facilitate cloning into the mammalian expression vector, pQE-31 (Qiagen, Valencia, CA). PCR products were blunt-end ligated into the pGEM T-Easy vector, and the vector transfected into E. coli DH5. Bacterial colonies were isolated and sequenced at the University of Alabama at Birmingham Genomics Core Facility to identify Pex5S. The Pex5S cDNA was removed from the pGEM vector by digestion with XmaI and SalI and ligated into pQE-31, which was digested using the same restriction sites. E. coli DH5 was transformed with the Pex5S-pQE vector, and Pex5S was expressed and purified in a similar manner as described for BAAT, with the exception of using a 0-500 mM NaCl gradient to elute the protein. Fractions containing Pex5S were identified via immunoblot analysis using a goat anti-human Pex5 antibody, and fractions containing high levels of Pex5S were pooled and stored at 70°C until used.

Immunoprecipitation
To immunoprecipitate Pex5, recombinant Pex5S protein (alone or in the presence of recombinant BAAT protein) was incubated for 3 h at 4°C with Protein A Sepharose beads bound to a goat anti-human Pex5 antibody, according to the ExactaCruz protocol. After incubation, the beads were washed three times by resuspension in 500 l 1× PBS followed by centrifugation at 5,000 g before being dissolved in 2× Laemmli sample buffer. The samples were boiled for 5 min before undergoing SDS-PAGE analysis.

BAAT activity radioassay
BAAT amidation activity was determined using the radioassay described by Johnson, Barnes, and Diasio (16) Reactions were initiated by the addition of cholyl-CoA, incubated at 37°C for 15 min, and terminated by addition of 0.5 ml 100 mM sodium phosphate (pH 2.0) containing 1% SDS. Radioactive conjugates were then extracted from unreacted labeled AA with water-saturated n-butanol and quantified by scintillation spectroscopy. Cholyl-CoA was synthesized from cholic acid and CoA by the method of Shah and Staple (17), with modifications as described previously (18).

LC/ESI/MRM for measurement of BAAT activity
Our laboratories have previously described a procedure to simultaneously measure BAAT N-acyltransferase and thioesterase activity (3). Thioesterase activity is measured by the accumulation of free bile acid after BAAT cleaves the CoA moiety from the bile acid-CoA thioester. The N-acyltransferase (amidation) activity is a measurement of the bile acid amidate formed after the conjugation of the bile acid with an AA. A reaction mixture was prepared containing potassium phosphate buffer (pH 8.4), 250 M taurine, and 60 M purified cholyl-CoA. The reactions were initiated by the addition of the appropriate BAAT protein and incubated for 15 min at 37°C. The reactions were terminated by addition of 60 l of 100% methanol per 100 l reaction volume. Internal standards were then added to aliquots of reaction mixture and stored at 20°C prior to LC/ESI/ MRM MS analysis. Because taurine was used as a substrate, the of carboxy-terminal mutations to the active site His-362 in BAAT (3). BAAT is capable of catalyzing both N-acyltransferase and thioesterase reactions (3); therefore, simultaneous measurement of both activities was performed using wild-type BAAT, BAAT-S, BAAT-SKL, BAAT-12AA Trunc, and BAAT-Ex1Del. BAAT-SKL was found to have greater than a 2.5-fold increase in N-acyltransferase activity compared with wild-type BAAT using cholyl-CoA and taurine as substrates ( Fig. 2A). Deletion of the final amino (N)-terminal truncation of BAAT (hBAAT-Ex1Del) was made by deleting the AAs encoded by the first exon (147 AAs) to investigate the possibility that a PTS sequence is located near the N terminus (Fig. 1).

Mutations in the C-terminus of hBAAT affect enzyme activity
Investigation into the effect of these mutations on enzymatic activity was warranted due to the close proximity of free cholic acid, BAAT-SKL showed a 78% increase as compared with wild-type BAAT (Fig. 2B). BAAT-S and BAAT-12AA Trunc also had thioesterase activities similar to wild-type BAAT. In contrast, BAAT-Ex1Del showed a 31% increase in thioesterase activity compared with wildtype BAAT (Fig. 2B).
two AAs in BAAT-S did not significantly alter N-acyltransferase activity as compared with wild-type BAAT. In contrast, BAAT-12AA Trunc and BAAT-Ex1Del possessed no detectable N-acyltransferase activity, although both enzymes still showed thioesterase activity ( Fig. 2A). When thioesterase activities were measured by the production determined for both taurine and glycine with BAAT-SKL were approximately 50% lower (Fig. 3, Table 1). The V max for N-acetyltransferase activity catalyzed by BAAT-SKL was about 2.5-fold greater than that for wild-type BAAT. Furthermore, the comparison of specific activity versus cholyl-CoA concentration demonstrated BAAT-SKL activity was not saturated until the cholyl-CoA concentration reached 300 M, as compared with wild-type BAAT that is saturated at a cholyl-CoA concentration of 50 M (Fig. 3A).

Substrate analysis of BAAT mutants
Kinetic characterization of BAAT-SKL indicates that small sequence changes in the PTS-1 significantly change the affinity of the enzyme for glycine and taurine, but not cholyl-CoA. Enzymatic studies have demonstrated that few AAs can be used as substrates for BAAT, with the most common in mammals being taurine, glycine, or both, depending on the species (13,(20)(21)(22). Our laboratories have previously reported that human BAAT can also use 2-fluoro--alanine as a substrate, but not -alanine (23). If BAAT-SKL has greater affinity for the known substrates glycine and taurine than wild-type BAAT, it is possible that it will accept other potential AAs as substrates. To determine whether mutating the carboxy-terminus of BAAT results in the ability to utilize other substrates, compounds structurally related to taurine and glycine were tested as substrates. The compounds tested were -alanine, D-alanine, L-alanine, 3-amino-1-propanesulfonic acid (homotaurine), and amino-methanesulfonic acid (nortaurine) (Fig. 4). Reaction products catalyzed by the different BAAT mutants were analyzed qualitatively by LC/ESI/ MRM MS to detect formation of cholate conjugates. A positive identification of a substrate for BAAT was made when a peak was apparent at the appropriate molecular mass (atomic mass units) indicating the addition of the compound to cholic acid minus the molecular mass of water. No peak was detected at 478.6 m/z when L-alanine was used in the assay in the presence of cholyl-CoA and either wild-type hBAAT or hBAAT-SKL (Fig. 5A). When alanine was incubated with cholyl-CoA in the presence of hBAAT-SKL, however, a peak was detected at 478.6 m/z, indicative of cholic acid conjugated with -alanine (Fig.  5B). In contrast, no peak was detected when -alanine was incubated with cholyl-CoA and wild-type BAAT (Fig. 5B). Both wild-type BAAT and BAAT-SKL were capable of conjugating nortaurine to cholic acid, although the activity of BAAT-SKL was apparently greater due to the disappearance of cholyl-CoA (m/z 578.3) (Fig. 5C). For homotaurine, wild-type BAAT had very little conjugating activity

Kinetic analysis of C-terminal hBAAT mutations
Because mutation of the carboxy-terminus of BAAT resulted in changes in amidation activity, the kinetic parameters of the N-acyltransferase reaction were investigated. The amidase reaction involves two substrates, and changes in activity could be due to an increase in cholyl-CoA binding, an increase in cholyl-CoA turnover, or an increase in the affinity for the AA substrate. The concentration of cholyl-CoA is also important to the reaction, as previous kinetic analysis of the reaction involving the conversion of cholyl-CoA to taurocholate or glycocholate by BAAT suggests a Tetra Uni ping-pong mechanism (20). Therefore, three Michaelis constants (K m ) were determined for each form of BAAT (hBAAT, hBAAT-S, and hBAAT-SKL) demonstrating N-acyltransferase activity: one for cholyl-CoA with taurine as substrate, one for glycine, and one for taurine.
Wild-type BAAT and BAAT-S had similar K m and V max values using cholyl-CoA and glycine or taurine as substrates (Fig. 3, Table 1). Wild-type BAAT and BAAT-SKL also had similar K m s for cholyl-CoA; however, the K m values Wild-type BAAT, BAAT-S, and BAAT-SKL were expressed in E. coli and purified via DEAE column chromatography. The rates of bile acid conjugate formation were measured by radioassay using radioactive AA. Fig. 4. Amino acid substrate candidates for hBAAT mutants. Representative chemical structures for possible AA substrates for wild-type hBAAT, hBAAT-S, and hBAAT-SKL. Glycine, taurine, and fluoro--alanine have previously been shown to be substrates for hBAAT (23). C-terminus of BAAT has a significant influence on the thermostability of the enzyme in vitro.

Pex5 interacts with BAAT and inhibits activity
When the expressed BAAT mutants were resolved by SDS-PAGE, BAAT-12AA Trunc and BAAT-Ex1Del exhibited electrophoretic mobilities different from the other forms of BAAT (Fig. 7A). To determine whether Pex5 and the BAAT mutants could interact in vitro, purified wildtype BAAT and the four BAAT mutants were incubated with purified recombinant human Pex5S for 1 h at 37°C, then immunoprecipitated with the goat anti-Pex5 antibody. The precipitates were then immunoblotted with the rabbit anti-BAAT antibody. Wild-type BAAT, BAAT-S, BAAT-SKL, and BAAT-Ex1Del all immunoprecipitated after incubation with Pex5S using the goat anti-Pex5 antibody, whereas hBAAT-12AA Trunc did not (Fig. 7B).
Because Pex5 can bind to BAAT, apparently at the carboxyterminus, and the last 12 AAs of the carboxy terminus are necessary for N-acyltransferase activity, Pex5 binding was evaluated for a role in the inhibition of N-acyltransferase with cholyl-CoA (expected ion at m/z 528.3). In contrast, BAAT-SKL completely converted cholyl-CoA to its homotaurine conjugate, as judged by the absence of the m/z 578.3 ion. These results indicate that hBAAT can use nortaurine as a substrate, whereas hBAAT-SKL can use nortaurine and homotaurine, as well as -alanine, as substrates. Nonetheless, neither wild-type BAAT nor BAAT-SKL could utilize L-alanine as a substrate to conjugate cholyl-CoA.

Thermostability of BAAT in vitro and in vivo
Thermal inactivation of enzymatic activity can reflect changes in the structure of proteins and the stability of the tertiary structure. To discern the role of the carboxy terminus of BAAT in the stability of N-acetyltransferase activity, purified BAAT, BAAT-S, and BAAT-SKL were incubated for increasing lengths of time at 42°C before measuring N-acyltransferase activity. BAAT-S showed a significant increase in the loss of N-acetyltransferase activity as compared with wild-type BAAT (Fig. 6). In contrast, hBAAT-SKL possessed a greater resistance to thermal inactivation than wild-type BAAT (Fig. 6). These results suggest that the present in peroxisomes to amidate the newly formed bile acids. Bile acids that have been deamidated by bacteria in the gastrointestinal tract before reabsorption are efficiently removed by hepatocytes from portal blood and amidated in the cytosol prior to being secreted into the biliary system (1).
Interaction of proteins with a PTS-1 sequence with Pex5 is involved in the translocation of many proteins into peroxisomes (24). The typical high activity PTS-1 sequence is -SKL. However, BAAT possesses a degenerate PTS-1 sequence at its carboxy terminus that may be involved with the differential distribution of BAAT between the cytosolic and peroxisomal compartments. A lower affinity for Pex5 binding may allow BAAT to be out-competed for Pex5 binding by proteins with canonical PTS-1 sequences. This lower affinity for Pex5 is consistent with the greater ability of the BAAT-SKL mutant to inhibit BAAT activity, indicating a greater affinity.
Interaction of the BAAT-SKL mutant with Pex-5 demonstrated the most inhibition of BAAT activity. The inhibition of wild-type BAAT and BAAT-S by Pex5 were similar, indicating that both proteins were interacting with Pex5 in an equivalent manner, even though the PST-1 sequence was deleted in BAAT-S. The physical interaction of BAAT, activity in the BAAT mutants. BAAT N-acyltransferase activity was measured after incubating purified wild-type BAAT, BAAT-S, and BAAT-SKL with varying levels of purified recombinant Pex5S. At equivalent ratios of Pex5 and BAAT, the amidation activity of BAAT-SKL was significantly more inhibited than the activity of wild-type BAAT or BAAT-S (Fig. 8). No significant difference was observed in the changes in amidation activity between wild-type BAAT and BAAT-S. Addition of DEAE-purified bacterial cytosol from cells transformed with an empty pQE31 vector or purified human SULT1E1 to wild-type and mutant forms of BAAT showed no effect on BAAT amidation activity (data not shown).

DISCUSSION
BAAT has the important function of forming bile acid N-acylamidates of both newly formed bile acids as well as those being returned to the liver after reabsorption from the gastrointestinal tract. To aid in this function, BAAT is localized both in peroxisomes and in the cytosol of hepatocytes (7)(8)(9)14). The final steps of bile acid synthesis occur in peroxisomes and approximately 25% of BAAT is   BAAT-SKL, and BAAT-S with Pex5 is supported by the observation that all three proteins were immunoprecipitated with Pex5 using the rabbit anti-BAAT antibody, and both wild-type and BAAT-S activity were inhibited by Pex5S. Because BAAT-12AA and Pex5 were not immunoprecipitated together, a relatively strong interaction is apparently occurring between Pex5 and the last 12 AAs of BAAT that does not require a PTS-1 sequence. Whether this interaction is sufficient to allow peroxisomal localization in cells remains unknown.
The carboxy-terminus of BAAT also has significant effects on the kinetic and substrate recognition properties of BAAT. The conversion of BAAT to BAAT-SKL resulted in a 2.5-fold increase in specific activity associated with a decreased K m for both taurine and glycine as substrates (Table 1). There was no effect on the affinity for cholyl-CoA. In contrast, the deletion of the final two AAs in BAAT-S did not alter the affinity for either glycine or taurine, indicating that the insertion of the Lys in the penultimate position was responsible for the increased amidation activity. The presence of the Lys also increased resistance of amidation activity to thermal inactivation, as compared with wild-type BAAT.
BAAT is capable of hydrolyzing the thioester linkage between cholate and the active site Cys (3). The alterations in the carboxy-terminus were also associated with changes in thioesterase activity. BAAT-SKL showed a 75% increase in thioesterase activity as compared with wild-type BAAT. The final 12 AAs of BAAT were required for amidase activity; however, BAAT-12AA Trunc that lacked amidase activity still possessed more thioesterase activity than wild-type BAAT. BAAT-ex1, which lacked 150 AAs at the amino terminus, was not capable of catalyzing amidase activity, but still possessed the same thioesterase activity as wild-type BAAT. The ability of BAAT to retain thioesterase activity while losing amidase activity may allow for mutations resulting in loss of bile acid amidate formation, while still catalyzing bile acid CoA hydrolysis.
Human BAAT has been reported to use a catalytic triad of Asp, His, and Cys to form bile acid amidates with the Cys being involved in the bile acid thioester linkage that is attacked by glycine or taurine to form the amidate (3). The sequence of the carboxy-terminal PTS-1 sequence had an unexpected effect on selectivity of the AA substrates. BAAT and BAAT-SKL were both capable of conjugating cholic acid with nortaurine and homotaurine; however, BAAT-SKL was also capable of using -alanine in the conjugation reaction. This indicates that the presence of the Lys in the SKL PTS-1 sequence increases accessibility to the cholic acid thioester linkage to substrates as well as water. However, the binding of cholyl-CoA and