Both maternal and offspring Elovl2 genotypes determine systemic DHA levels in perinatal mice[S]

The molecular details relevant to dietary supplementation of the omega-3 fatty acid DHA in mothers as well as in their offspring are not clear. The PUFA elongase, elongation of very long-chain fatty acid (ELOVL)2, is a critical enzyme in the formation of DHA in mammals. In order to address the question regarding the origin of DHA during perinatal life, we have used DHA-deficient Elovl2-ablated mice as a model system to analyze the maternal impact on the DHA level in their offspring of various genotypes. Elovl2−/− mothers maintained on control diet had significantly lower systemic levels of DHA compared with the Elovl2+/− and Elovl2+/+ mothers. Dietary DHA administration during the pregnancy and lactation periods led to increased DHA accretion in maternal tissues and serum of all genotypes. The proportion of DHA in the liver and serum of the Elovl2−/− offspring was significantly lower than in the Elovl2+/+ offspring. Remarkably, the DHA level in the Elovl2+/− offspring nursed by DHA-free-fed Elovl2−/− mothers was almost as high as in +/+ pups delivered by +/+ mothers, suggesting that endogenous synthesis in the offspring can compensate for maternal DHA deficiency. Maternal DHA supplementation had a strong impact on offspring hepatic gene expression, especially of the fatty acid transporter, Mfsd2a, suggesting a dynamic interplay between DHA synthesis and DHA uptake in the control of systemic levels in the offspring.

experiment. A few days prior to delivery, the animals were separated into single cages and, within 24 h after spontaneous delivery, litter size for each female was recorded. Pups were kept together with lactating mothers for about 2 weeks postpartum and no correction of the litter size was performed. At the end of the study, offspring were euthanized together with mothers or only the offspring were euthanized and / mothers were used for a subsequent breeding. Maternal body weight was measured at the start and at the end of the study and offspring body weight was recorded after they had been euthanized. A nonpregnant control group was composed of females that did not give birth. Mothers and control females were euthanized by CO 2 administration and cervical dislocation. Blood was collected by direct heart puncture. Offspring were euthanized by cervical dislocation and blood was collected after decapitation. All blood samples were kept at 4°C and thereafter centrifuged at 10,000 g at 4°C for 5 min and serum was collected. Each pup was genotyped postmortem and, due to small volumes, serum from single pups of each genotype was pooled. Tissues samples were dissected out from each animal and immediately put in liquid nitrogen and then stored at 80°C until being further analyzed. For a detailed experimental set-up, see supplemental Fig. S1. All animals were housed at room temperature, maintained on a 12 h light/dark cycle, had free access to water, and were fed ad libitum. All studies were carried out with ethical permission from the Animal Ethics Committee of the North Stockholm region, Sweden.

MRI measurement
In order to measure body fat and lean content of the offspring, in vivo MRI using EchoMRI-100 TM (Echo Medical Systems, perinatal development and does dietary supplementation matter? Until now, studies regarding DHA deficiency during perinatal development have been limited to maternal depletion of dietary omega-3 PUFAs. We have previously reported that ablation of the ELOVL2 enzyme in Elovl2 / mice leads to a systemic DHA deficiency by about 90% (21). By the use of our Elovl2 / animal model, we here bring novel data to the field by presenting results showing that endogenous PUFA synthesis in the offspring is the major source of DHA in neonates under normal conditions. We also present data on how dietary intervention during gestation and lactation affects the DHA status in both mothers and offspring under normal and genetically acquired DHA-deficient conditions.

Diet
Control diet (D12450H) and DHA-enriched diet (D13021002) were formulated in the form of food pellets by Research Diets, New Brunswick, NJ. Both diets were low-fat diets where the fat content was equal to 10% of calories ( Table 1). The DHA content in the DHA-enriched diet was 14.5% of total fatty acids and there was no trace amount of this fatty acid in the control diet ( Table 2). Moreover, both diets had a similar linoleic acid (18:2n-6)/ALA (18:3n-3) ratio of 8/1. Both diets were provided ad libitum during the whole time of the experiment. In order to reduce the risk of oxidation, new food pellets were provided every 7 days.  sizes above), and for comparisons of mothers and females, log2 absolute fold change was calculated. In addition, multiple linear regression models were fitted to assess the relationship between weight and groups adjusted by litter size. Pearson's correlation coefficient was calculated between DHA measurements across different tissues in both mothers and nonpregnant females or only across tissues in mothers. All the statistical significances are listed in supplemental Tables S2-S15.

Effects of female Elovl2 genotype and DHA supplementation on breeding outcome
It is well-known that the genotype of the female influences the possibility of being pregnant as well as the pregnancy outcome. In our experimental set-up (supplemental Fig. S1), breeding of wild-type females fed control diet resulted in 100% successful pregnancies, whereas Elovl2 +/ and Elovl2 / females showed, independently of the genotype of the male, a tendency toward, but not significantly, a lower pregnancy success rate ( Table 3). Likewise, litter size was somewhat, but not significantly, smaller for the Elovl2 +/ and Elovl2 / mothers than for the wild-type mother group (Table 3).
DHA supplementation did not affect the breeding outcome of Elovl2 +/+ and Elovl2 +/ female mice. However, the number of successful pregnancies was reduced for the Elovl2 / females and, even with a significantly high number of breeding pairs (21), Elovl2 / females fed DHAenriched diet mated with +/ males showed a significantly lower percentage of successful pregnancies in comparison with the other genotypes, which indicates an adverse dietary effect (Table 3). There were no statistical differences in pup survival or Mendelian distribution between the genotypes regardless of diet.

Effects of Elovl2 genotype and DHA supplementation on maternal and offspring body weight
Several studies have reported effects of DHA supplementation during pregnancy and lactation on the length of gestation and, consequently, the birth weight of the offspring. In the present study, the body weights of the mothers at postpartum stage tend to be higher than the weight of control females (Fig. 1A), as they gained weight throughout the gestation and lactation periods (Fig. 1B). There was no significant difference in the body weight between different genotypes and between dietary treatments (Fig. 1A). Maternal DHA supplementation did not show any significant effect on body weight of the suckling pups regardless of genotype ( Fig. 2A). Nevertheless, offspring delivered from +/+ mothers supplemented with DHA tended to have higher body weight than those that were supplied by mothers fed control diet ( Fig. 2A). Body composition measurements revealed that the observed difference in body weight could be due to a higher lean mass in those animals (Fig. 2B). However, when taking the contribution of litter size into consideration using multiple linear regression models, we were able to conclude that litter size significantly affects the body weight outcome (Pr = 0.0265). Houston, TX) was performed after the animals had been euthanized and liver and brain dissected out.

Fatty acid analysis
Tissues and diets were extracted with hexane-isopropanol (3:2, v/v), following the method of Hara and Radin (22). Total lipids in diets and tissues were methylated with boron trifluoride and dry methanol, following the procedure of Appelqvist (23) and the fatty acid methyl esters were analyzed with a gas chromatograph CP-3800 (Varian AB, Stockholm, Sweden) equipped with flame ionization detector and split injector and an autosampler mode (Combi PAL AutoSampler; Varian AB). The column was a fused silica capillary column BPX 70 (SGE, Austin, TX), length 50 m id. 0.22 mm, 0.25 m film thickness. The column temperature was programmed to start at 158°C and kept for 5 min and then increased by 2°C/min up to 220°C where it remained for 8 min. The carrier gas was helium (0.8 ml/min) and the makeup gas was nitrogen. The injector and detector temperatures were 230°C and 250°C, respectively. Fatty acids were identified by comparison with the standard fatty acid mixture, GLC-68 (Nu-check Prep, Inc., Elysian, MO), and retention times. Peak areas were integrated using Star chromatography workstation software version 5.5 (Varian AB). All solvents and other chemicals used for analysis were purchased from Merck and were used without further purification.

Quantitative RT-PCR
RNA was isolated from similar pieces of offspring livers with TRI Reagent (Sigma-Aldrich) following the manufacturer's procedure. For real-time PCR, 500 ng of total RNA for each sample was reverse transcribed using random hexamer primers, dNTPs, multiscript, and RNase inhibitor (Applied Biosystems, Foster City, CA). cDNA samples were diluted 1:10 and duplicate aliquots of 2 l of each sample cDNA were mixed with SYBR Green Jump-Start Taq ReadyMix (Sigma-Aldrich), prevalidated primers, and DEPC-treated water. PCR-primers were used for Fas, steroyl-CoA desaturase 1 (Scd1), sterol regulatory element-binding protein 1c (Srebp-1c), major facilitator superfamily domain containing 2a (Mfsd2a), Elovl2, Fads1, Fas, and Elovl5. For primer sequences, see supplemental Table S1. Expression analysis was performed using the Bio-Rad detection system. Data were normalized to the ribosomal 18S RNA.

Statistical analyses
All statistical analyses, except for mRNA analysis that was performed by using GraphPad PRISM (San Diego, CA), and the statistical differences were calculated with Student's unpaired t-test and were performed under R, a programming language and software environment for statistical computing and graphics. Pearson's chi-squared test was used to evaluate differences in successful pregnancies and offspring survival within and across different genotypes. Kruskal-Wallis rank sum test was used to test differences between litter sizes. The P values were adjusted for multiple comparisons using the Benjamini-Hochberg method, and P < 0.05 was used as a significance cut-off. Principal component (PC) analysis (PCA) was used to visualize the high dimensional data on the two-component space, along PC1 and PC2, which explains the most variance in the data. The consensus dataset was prepared prior to the PCA by including fatty acids (P = 30) with complete measurements across all the tissues and samples (n = 199). The ellipses on the PCA score plots were drawn at 75% confidence level and the loading plots are based on the absolute loading values. The differences in DHA level in tissues between the groups and within the same genotype were assessed by nonparametric test, Kruskal-Wallis rank sum test (as for differences between litter

Effects of Elovl2 genotype on accretion of DHA in maternal tissues and serum throughout pregnancy and lactation
Liver, white adipose tissue (WAT), mammary glands, and serum were isolated from mothers 2 weeks after delivery. Lipid weight obtained from each sample was normalized to tissue wet weight and expressed as percent lipids for each analyzed sample. There was no significant difference in the relative amount of extracted lipids between the various genotype groups or across the dietary treatments within each tissue, with the highest level from WAT (approximately 50%), followed by mammary glands (8%), liver (5%), and serum (0.5%) (supplemental Fig. S2A-D). In addition, we observed a higher level of extracted lipids from mammary glands from control females than from mothers (supplemental Fig. S2C). The relative amount of extracted lipids from offspring liver and serum (supplemental Fig. S2E, F) resembled the amount obtained from corresponding / (/) mothers and control females fed either control diet (Control) or DHA-enriched diet (DHA). Results are presented as mean ± SEM of two to five mice. Statistical significances are shown between groups, ***P < 0.001.  was significantly reduced in Elovl2 / mothers fed control diet and the level increased considerably upon dietary supplementation (Fig. 3C). As for the other tissues, Elovl2 / mothers exhibited a higher level of DHA accretion in mammary glands than Elovl2 +/ or Elovl2 +/+ mothers (Fig. 3C). Although DHA supplementation augmented the DHA level in mammary glands of control females, the level was markedly lower than in maternal tissues regardless of genotype.
The levels of DHA in WAT of mothers of all genotypes fed regular diet was almost undetectable (Fig. 3D). However, DHA supplementation elevated the proportion of DHA, especially in Elovl2 / mothers. In control females, we observed a significant increase of DHA also in WAT of Elovl2 +/+ mice (Fig. 3D).
When we performed DHA scatter plots across different tissues of mothers and control females of all genotypes, we found a high Pearson correlation between the percentage of DHA in the liver and serum (0.90) (Fig. 4A), liver with mammary glands (0.72) (Fig. 4B), and serum with mammary glands (0.57) (Fig. 4C). When samples from control females were excluded, the correlation coefficient became stronger between mammary glands and liver (0.92) and between mammary glands and serum (0.92), which clearly indicates that the Elovl2 genotype affects DHA accretion in mothers in a systemic manner during pregnancy and lactation. Instead, the proportion of DHA in mammary gland of control females seemed to have a higher correlation coefficient with WAT (Fig. 4D). In contrast, a low correlation was found between WAT and liver or serum in any of the females (Fig. 4E, F).

Effects of Elovl2 genotype on fatty acid composition in mothers and nonpregnant females
The complete analysis of fatty acid composition of liver, serum, mammary glands, and WAT is presented in supplemental Tables S16-S19, respectively. However, because we expressed the DHA proportion as a percentage of the total fatty acids for a given tissue, we did not analyze differences within/between other fatty acids because these might be considered as potential confounders in regard to total fatty acid composition. Although, when we performed PCA, loading plots indicated that the main fatty acids contributing to PC1 were 18:1n-9, 22:6n-3, 20:4n-6, and 18:2n-6 (supplemental Fig. S3A) and for PC2, it was mainly 16:0, 18:2n-6, 20:4n-6, and 14:0 (supplemental Fig. S3B).
PCA of all the tissue samples showed a distinct fatty acid composition within each tissue [female WAT, serum, mammary glands, and liver, and offspring liver (supplemental Fig. S3C)], as well as in total fatty acid composition between mothers and control females (supplemental Fig. S3D).
Samples from mammary glands (green) were split into two subgroups along PC2 (supplemental Fig. S3C), where one subgroup was different from the other tissues (PCA scores exclusively from the mothers dataset), while the other subgroup exclusively overlapped with WAT (PCA scores for both mothers and nonpregnant females dataset) supporting the correlation data in Fig. 4 that the fatty acid content of the maternal mammary glands resembles both WAT maternal tissues and, similarly, did not differ between genotypes or maternal diet.
When we analyzed specific fatty acid levels, the Elovl2 / mothers showed significantly reduced DHA levels in liver and serum compared with Elovl2 +/+ and Elovl2 +/ mothers fed control diet (Fig. 3A, B). There was no significant difference in DHA levels between mothers and control females of the same genotype (Fig. 3A, B). DHA supplementation resulted in increased DHA concentration in both mothers and nonpregnant females of all genotypes. Interestingly, Elovl2 / mothers tended to accumulate more DHA than the Elovl2 +/+ and Elovl2 +/ mothers. This high accretion of DHA was not observed in Elovl2 / control females fed DHA-enriched diet (Fig. 3A, B). Consistent with liver and serum, the level of DHA in mammary gland compared with +/+ pups delivered by +/+ mothers, but the level was significantly higher than in / pups without any maternal DHA input (Fig. 5A). Analysis of pooled serum samples from the same groups of offspring showed the same relationship of DHA deposition as in the liver (Fig.  5B) implying that offspring liver, like in adult mice, is the major contributor of systemic DHA under standard dietary conditions and that, at least before weaning, endogenous DHA synthesis in the pups can almost fully compensate for maternal DHA deficiency. Interestingly, independently of maternal or offspring Elovl2 genotype, maternal dietary DHA supplementation during the gestation and lactation periods resulted in an increased proportion of DHA up to 20% of total fatty acids in the liver (Fig. 5A) and 10% in serum (Fig. 5B) of all offspring groups. When we performed PCA score plots on total fatty acid composition, offspring liver samples showed clear differences between samples coming from pups supplied by mothers fed a DHA-enriched diet and those that were delivered by mothers kept on a control diet and liver signatures; whereas in control females, it resembles only the WAT signature.

DHA levels in offspring liver and serum in regard to maternal and offspring Elovl2 genotype
Regarding the contribution of maternal and offspring Elovl2 genotypes on perinatal DHA accretion during pregnancy and lactation, we analyzed eight distinct groups of pups of particular genotype (Elovl2 +/+ , Elovl2 +/ , and Elovl2 / ) delivered by mothers of certain genotype (Elovl2 +/+ , Elovl2 +/ , and Elovl2 / ) fed control or DHA-enriched diet. The complete analysis of the fatty acid composition of liver and serum is presented in supplemental Tables S20 and S21, respectively. We could observe that the proportion of DHA in the liver of wild-type offspring delivered by wildtype mothers accounted for 10% of total fatty acids, which was significantly higher than in the Elovl2 / neonates coming from Elovl2 +/ mothers (4%) and Elovl2 / mothers (1%) (Fig. 5A). Heterozygote +/ pups from DHA-deficient / mothers showed slightly reduced DHA levels (7%) For the percent of remaining fatty acids, see respectively, supplemental Tables S16-S19. Results are presented as mean ± SEM of two to five mice. #Represents a log2 absolute fold change >1 between control (Control) and DHA-enriched (DHA) diet, unless specified.

pups fed by Elovl2
/ mothers maintained on control diet (Fig. 6C, D). Maternal DHA supplementation led to a downregulation of Fads1 and Fads2 in all genotypes (Fig.  6C, D); whereas, Elovl2 and Elovl5 were only downregulated in +/+ neonates fed by +/+ mothers and Elovl5 also when fed by +/ mothers. Conclusively, the hepatic expression of the PUFA enzymes in the offspring is regulated in a noncoherent manner and affected not only by the Elovl2 genotype but also by the amount of maternal DHA supply. Moreover, the offspring of mothers maintained on a DHAenriched diet showed a significant downregulation of the classical lipogenic transcription factor, Srebp-1c, and its target genes, Fas and Scd1 (Fig. 7A-C), which supports the concept of DHA as a suppressor of lipogenesis described previously in adult animals (25)(26)(27). Because our data suggests that the DHA level in the offspring is a result of a dynamic interplay between hepatic (supplemental Fig. S4), suggesting that maternal DHA supplementation has noticeable effect on offspring total fatty acid composition.

Effect of maternal and offspring genotype and maternal diet on offspring hepatic gene expression
In order to see whether the DHA levels in the offspring groups described in Fig. 5 correlated with hepatic gene expression, we analyzed mRNA levels of genes regulating PUFA synthesis in the liver of the offspring. The levels of Elovl2 expression confirmed the genotypes of the offspring and indicated, as previously reported (24), a haploinsufficiency by 50% in heterozygote (Elovl2 +/ ) offspring (Fig.  6A). Interestingly, we did not observe any significant upregulation of Elovl5 as a compensatory mechanism for the loss of Elovl2 (Fig. 6B). The expression of Fads1 and Fads2 showed a tendency to be higher especially in the Elovl2 / Mfsd2a expression complements hepatic DHA synthesis to balance the level of DHA in the offspring.

DISCUSSION
To fulfill the physiological demands of the offspring, researchers have previously pointed out the importance of high systemic levels of the omega-3 fatty acid DHA in females during their reproduction cycle. In this study, we investigated the role of ELOVL2 and PUFA synthesis in establishing physiological levels of DHA in pregnant and lactating mice, as well as in their offspring. For this we used Elovl2-ablated (Elovl2 / ) mice, which previously have been shown to have severely reduced DHA levels to about 10% of normal levels in serum (21), and analyzed fatty acid levels in various tissues and serum in lactating mothers and their offspring, as well as in nonpregnant females. Our results show that both endogenous synthesis and maternal provision of DHA are required sources for obtaining normal systemic DHA levels in the offspring during pregnancy and lactation. However, in contrast to common beliefs, we can show that before weaning, endogenous DHA synthesis in the offspring can almost fully compensate for maternal DHA deficiency. Our data also imply that endogenous PUFA synthesis in mothers cannot fully compensate for deficient synthesis in mice during perinatal life.
DHA levels during pregnancy and lactation tend to show a quite dynamic pattern for both mothers and their offspring. During the first two thirds of pregnancy, there is an observed increase of DHA levels in human serum that is a result from increased endogenous synthesis as well as mobilization from internal stores (4,28). A recent study shows that DHA levels can start to increase as early as 29 days post luteinizing hormone intervention (29). However, in late pregnancy and after parturition, a stage of DHA reduction has been shown to occur in human plasma (30). This period correlates with increased demands of DHA supply into the fetus or neonates for, e.g., optimal brain development (2). In breastfeeding mothers, up to a 30% depletion in the maternal circulation level of DHA has been observed (31), which may explain why we did not observe higher DHA levels in lactating mothers compared with the control females. The PCA scores revealed a clear distinction in fatty acid composition between mothers and control females, which implies maternal adipose depletion in mammary glands due to lactation. To fully understand the role of ELOVL2 and DHA synthesis during fetal and neonatal life it would therefore be valuable to monitor the DHA status in serum in our experimental groups during different time points throughout the pregnancy and lactation periods.
Several population studies have pointed out an association between human carriers of Fads1 and Fads2 haplotypes and the DHA level in pregnant and lactating mothers, as well as in their offspring (32)(33)(34). Although our data here imply that gene variants of Elovl2 can contribute to differences in the maternal DHA pool in mammals, a limited number of studies have pointed out a role for Elovl2 during pregnancy and lactation. Rodriguez-Cruz and coworkers have reported that Elovl2 upregulation in the maternal rat DHA synthesis and DHA uptake, we examined to determine whether the Elovl2 genotype and the level of maternal DHA supply during pregnancy and lactation may affect the hepatic expression of the DHA transporter, Mfsd2a. As seen in Fig. 7D, Mfsd2a was significantly expressed in all offspring groups and there was a tendency of increased expression, particularly in Elovl2 / neonates fed by Elovl2 +/ mothers kept on control diet, in both +/ and / offspring groups (Fig. 7D). Remarkably, maternal dietary DHA supplementation significantly downregulated the expression of the transporter in all groups, suggesting that  Tables S20, S21. Results are presented as means ± SEM of two to five mice (A) or as pooled samples from three to five individuals (B). *Represents statistical significance (P < 0.05) between genotypes; #Represents statistical significance (P < 0.05) between control (Control) and maternal DHA-enriched diet (DHA). genotype, which implies the existence of a plausible set-point for how much DHA can be accreted. In addition, Elovl2 / mothers fed a DHA-enriched diet exhibited accumulation of DHA, for all types of analyzed tissues, much over the level of mothers of other genotypes, as well as the control females of the same genotype. This may indicate the existence of a specific compensatory mechanism in response to DHA deficiency during pregnancy and lactation for Elovl2 / mothers when DHA is available in the diet. Moreover, the relative amount of DHA in the offspring liver reached a relatively higher level than in the maternal liver, which is a good illustration of a bio-magnification phenomenon (43). Although there has been a growing interest in the field of diet-genotype interplay and body DHA levels (44), to our knowledge, this is the first report that focuses on the effect of maternal DHA supplementation in a condition of genetically acquired systemic DHA-deficiency and liver at 20 days of pregnancy can contribute to increased long-chain PUFA synthesis and DHA levels during late pregnancy and lactation (35). Moreover, the same group observed an increased Elovl2 expression in adipose tissue within the first half of the lactation period (36), suggesting improved mobilization of DHA from internal sources. There are also reports regarding low levels of Elovl2 mRNA and protein in placenta and in mammary glands during lactation (35)(36)(37)(38).
Several studies have implied that maternal DHA supplementation may rescue DHA depletion during pregnancy and lactation and lead to a successful supply of DHA to the offspring (30,(39)(40)(41)(42). In agreement with this, maternal DHA supplementation increased the level of DHA in all tissues of mothers and control females, as well as in the offspring. Interestingly, the DHA level in the pups reached a ceiling of about 20% of total fatty acids regardless of Results are presented as mean ± SEM of two to five mice. *P < 0.05, **P < 0.01, ***P < 0.001.
of gestation and, consequently, the birth weight of the offspring (17,51,52). In the present study, DHA supplementation seemed to increase offspring body weight, which was linked to increased lean weight, but not fat mass. This suggests that, before weaning, DHA modulates growth and developmental features rather than the fat metabolism (53). However, we did not record the occurrence of vaginal plug and therefore the precise length of gestation was not monitored nor did we make any adjustments for litter size, which both may have a strong impact on body weight and composition.
In line with the previous reports on fetuses and neonates (11,13), we detected relatively high expression of genes involved in PUFA synthesis (Elovl5, Fads1, and Fads2) in the liver of suckling mice. Although experimental data suggest that the activity of the PUFA enzymes is mainly controlled at the transcriptional level, Elovl2-ablation in the offspring not by dietary manipulation such as depletion of DHA or its precursors, such as ALA.
Previous studies have revealed that FADS2-ablation or dietary DHA deficiency affects fertility of both genders, as well as breeding outcomes in general (45)(46)(47)(48). In contrast to this, we observed that Elovl2 / mothers fed a DHA-enriched diet had a lower level of successful pregnancies (Table 3). Regarding the Elovl2 / genotype, which is a model system reflecting insufficient conversion of EPA into DHA, we observed relatively high maternal levels of EPA under standard conditions, which upon dietary supplementation, became even higher (supplemental Tables S16-S19) and may have a negative effect on pregnancy outcome (49,50). Nevertheless, this hypothesis needs to be supported by further and more detailed studies.
Several studies have reported effects of DHA supplementation during pregnancy and lactation on the length +/ (+/), and Elovl2 / (/) genotype delivered by Elovl2 +/+ (+/+), Elovl2 +/ (+/), and Elovl2 / (/) mothers fed either control diet (Control) or DHA-enriched diet (DHA) expressed as relative mRNA levels (target gene/18S). Results are presented as mean ± SEM of two to five mice. *P < 0.05, **P < 0.01, ***P < 0.001. did not lead to a general compensatory upregulation of any of the genes. Recently, Park et al. (54) reported that Fads2 transcript in human cells can catalyze 4-desaturation and be actively involved in direct synthesis of DHA from 22:5n-3. While we did not detect any effects on the DHA level in Elovl2 / pups fed by Elovl2 / mothers, the maternal genotype appeared to have a significant impact on the expression levels of the desaturases, but not on Elovl5.
An interesting observation was that the expression levels of Mfsd2a, which is identified as a transporter of DHA as lysophosphatidylcholine-DHA (55) and is significantly expressed in liver, presented a mirror image of the DHA levels found in offspring liver and serum and was almost undetectable in the groups fed by mothers on a DHAenriched diet, implying a negative feed-back mechanism when the DHA levels have reached a ceiling in the offspring. This is in line with previous results indicating that Mfsd2a is a nutritionally regulated gene that plays numerous roles in body growth and development, motor function, and lipid metabolism (56,57). Its expression in liver is found to be dependent on PPAR and glucagon signaling and is increased in liver upon food deprivation. Accordingly, Mfsd2a has therefore been suggested to play a role in adaptation to fasting. However, to our knowledge, this is the first in vivo study on the regulation of Mfsd2a regarding DHA deficiency and DHA supplementation.
In conclusion, analysis of DHA levels in the diverse Elovl2 genotypes of mothers and their offspring, gave us a possibility to address questions concerning the source of DHA during perinatal life. Combining our findings, we can conclude that both maternal and offspring Elovl2 genotypes, as well as maternal dietary supplementation, contribute to DHA accretion during perinatal life and are controlled by a dynamic interplay between hepatic DHA synthesis and DHA uptake in the offspring (Fig. 8). Undoubtedly, additional precise investigations are required regarding DHA sources and offspring fatty acid deposition during the whole pregnancy and lactation period.