Secretory phospholipase A2 group IIA modulates insulin sensitivity and metabolism

Secretory phospholipase A2 group IIA (PLA2G2A) is a member of a family of secretory phospholipases that have been implicated in inflammation, atherogenesis, and antibacterial actions. Here, we evaluated the role of PLA2G2A in the metabolic response to a high fat diet. C57BL/6 (BL/6) mice do not express PLA2g2a due to a frameshift mutation. We fed BL/6 mice expressing the human PLA2G2A gene (IIA+ mice) a fat diet and assessed the physiologic response. After 10 weeks on the high fat diet, the BL/6 mice were obese, but the IIA+ mice did not gain weight or accumulate lipid. The lean mass in chow- and high fat-fed IIA+ mice was constant and similar to the BL/6 mice on a chow diet. Surprisingly, the IIA+ mice had an elevated metabolic rate, which was not due to differences in physical activity. The IIA+ mice were more insulin sensitive and glucose tolerant than the BL/6 mice, even when the IIA+ mice were provided the high fat diet. The IIA+ mice had increased expression of uncoupling protein 1 (UCP1), sirtuin 1 (SIRT1), and PPARγ coactivator 1α (PGC-1α) in brown adipose tissue (BAT), suggesting that PLA2G2A activates mitochondrial uncoupling in BAT. Our data indicate that PLA2G2A has a previously undiscovered impact on insulin sensitivity and metabolism.

In addition to its inflammatory properties, PLA2G2A is proatherogenic with elevated levels of PLA2G2A being a biomarker for cardiovascular disease (16). Evidence of a role for PLA2G2A in atherosclerosis came from work utilizing PLA2G2A transgenic mice. C57BL/6 (BL/6) mice do not express PLA2g2a due to frameshift mutation in exon 3. The human PLA2G2A gene was introduced into BL/6 mice to create PLA2G2A-expressing (IIA+) mice (17,18). When fed a high cholesterol diet, the IIA+ mice had increased atherosclerotic lesions, reduced plasma HDL, and slightly increased LDL (17). They also had elevated hepatic cholesterol levels, suggesting that PLA2G2A affects hepatic cholesterol uptake (19). Furthermore, bone marrow transplantation from IIA+ mice into LDL receptor-deficient mice increased atherosclerotic lesions, and expression of PLA2G2A in mouse macrophages accelerated the development of arterial wall lesions and the movement of cholesterol from LDL to foam cells (20,21). Oral dosing of varespladib, an inhibitor of sPLA2g2a, for 16 weeks reduced aortic atherosclerosis in ApoE-deficient mice (22). However, the failure of varespladib to reduce myocardial infarction and cardiovascular death in clinical trials has called into question the view that PLA 2 is pro-atherogenic (23,24).
There is a relationship between PLA2G2A, inflammatory processes, and cardiovascular disease. However, the contributions of PLA2G2A to metabolic regulation have not been examined. In this work, we investigated the metabolic effects of PLA2G2A in relation to hypercaloric high fat feeding. Our findings demonstrated that IIA+ mice are resistant to body weight and fat mass gains in response to a high fat diet. Moreover, IIA+ mice have superior glucose tolerance and insulin sensitivity, and these changes are associated with elevations in total energy expenditure. This study has identified a novel role of PLA2G2A in the regulation of whole-body metabolism.

IIA+ mice
The IIA+ mice, which express the human PLA2G2A gene under the regulation of the human promoter, were provided by Dr. Eric Boilard at the Université Laval (CHUL), Quebec, Canada (25). Male mice heterozygous for the PLA2G2A gene were bred with BL/6 females. For these experiments, male BL/6 and IIA+ mice were used. Mice were placed on diets 8 weeks after birth. In each dietary group, we had 11 BL/6 chow-fed mice, 10 IIA+ chow-fed mice, 11 BL/6 high fat diet-fed mice, and 12 IIA+ high fat diet-fed mice. Mice were housed with a constant light and dark phase of 12 h at 20-23°C. All animal procedures were approved by the University of Tennessee Health Science Center Institutional Animal Care and Use Committee.

Diet and food intake
The chow diet was the Teklad LM-485 mouse/rat diet (7012) and the high fat diet was D12451 from Research Diets Inc. The high fat diet provided 45% of the calories from fat, while the chow diet contained 5% of the calories as fat. The mice were given free access to food throughout the study. The food was weighed at weekly intervals and the kilocalorie consumption was calculated based on grams of food eaten, the calorie density of the food, and the weight of the mice.

Body composition analysis
Mice were weighed and total fat and fat-free mass were determined weekly by EchoMRI-1100.

Glucose and insulin tolerance tests
Following 6 h fasts, baseline blood glucose concentrations were determined in blood collected from a small tail incision using a hand-held glucometer (AccuCheck). D-glucose in PBS [2 mg/kg fat-free mass; glucose tolerance test (GTT)] or insulin [1 U/kg fatfree mass; insulin tolerance test (ITT)] was injected intraperitoneally and blood glucose concentrations measured at 15,30,45,60,75,90,105, and 120 min postinjection (26). The data are presented as blood glucose levels versus time and the area under the glucose curve.

Comprehensive laboratory animal monitoring system
After 11 weeks of diet, mice were individually housed in the comprehensive laboratory animal monitoring system (CLAMS) chambers. They were maintained on either chow or high fat diet. Total energy expenditure was determined using indirect calorimetry (27). VO 2 and heat production are expressed relative to fatfree mass. The respiratory exchange ratio (RER) was measured to determine energy substrate preference. Physical activity was determined by the number of infrared beam breaks.

RNA extraction and real-time PCR
Mice were allowed access to food prior to tissue collection for RNA and protein. The mice were anesthetized by isoflurane and then euthanized by cervical dislocation. Liver, quadriceps muscle, brown adipose tissue (BAT), and epididymal white adipose tissue (eWAT) were harvested and stored in liquid nitrogen. Total RNA was extracted from mouse liver by RNA-STAT 60 (Tel-Test). cDNA was synthesized via reverse transcription using Superscript III (Invitrogen). The parameters for RT-PCR were as follows: 95°C for 5 min, 40 cycles of 95°C for 15 s, 60°C for 30 s, and 72°C for 10 s. Cyclophilin D was used as a reference gene. The quantification of the PCR products was carried out using the Ct method (28).
The forward and reverse primers used for real-time PCR are shown in Table 1. The PLA2G2A primers were from Qiagen (catalog number PPH05823B).

Western blots
Total protein from mouse livers, quadriceps, BAT, and eWAT was isolated in RIPA buffer containing protease inhibitors [50 mM Tris-HCl (pH 7.4), 100 mM NaCl, 5 mM EDTA (pH 8.0), 1% Triton, 1 mM benzamidine, and 0.5 mM PMSF] (29). Protein lysates were prepared in loading buffer and equal amounts were loaded on 4-20% gradient Tris/glycine precast acrylamide gels. Proteins were separated using SDS-PAGE and transferred to nitrocellulose membranes. Membranes were blocked with 5% nonfat milk in TBS-T prior to antibody incubation. Membranes were immunoblotted with the appropriate primary antibody in 5% BSA in TBS-T and secondary antibody in 5% nonfat milk in TBS-T. Immunoreactive proteins were detected using Super Signal West Femto chemiluminescent substrate (Thermo Scientific) (28

Lipids
Plasma cholesterol and lipoproteins in serum were measured at the University of Tennessee Endocrinology/Lipoprotein Laboratory. Blood was obtained by cardiac puncture from anesthetized mice and the serum was collected from the clotted blood. For hepatic triglyceride measurements, liver samples were first lysed in homogenization buffer [50 mM Tris (pH 8), 5 mM EDTA, 30 mM EDTA, 30 mM mannitol, and phosphatase inhibitors]. Lipids were then extracted with a chloroform/methanol mixture using the Folch extraction procedure (30). Once the chloroform phase was evaporated, hepatic triglycerides were Fig. 1. Weight gain and body composition of IIA+ mice on high fat diets. BL/6 and IIA+ mice were placed on chow diet or HFD for 10 weeks, as described in the Materials and Methods. A: The weight gain (grams) of the mice in the four groups was assessed on a weekly basis. B, C: MRI analysis was performed weekly and used to determine the lean mass and percent body fat. D: The average kilocalories consumed per mouse per gram of body weight were calculated from the food intake and the calorie content of the various diets. In all groups, there were 10-12 mice. The differences in the various time points were determined by two-way ANOVA, using genotype and diet as two separate factors. measured using the Sigma triglyceride assay kit as directed by the manufacturer.

Statistical analysis
Data analysis was performed using the JMP Statistical Discovery program. Data presented are the mean results of 3-4 replicate experiments ± the SE. Data were analyzed by Student's one/ two-tailed t-test or ANCOVA. P < 0.05 was considered to be statistically significant.

Effect of PLA2G2A on weight gain
Initially, we evaluated the effect of PLA2G2A on weight gain and lipid accumulation by monitoring the body composition in response to 10 weeks of high fat diet consumption in both BL/6 control and the IIA+ mice (15). In response to the high fat diet, BL/6 mice gained significantly more weight than the chow-fed BL/6 mice or the IIA+ mice on either the chow or high fat diet. The IIA+ mice were resistant to high fat diet-induced weight gain (Fig. 1A). The fat-free mass of BL/6 and IIA+ mice was similar on both the chow and high fat diets (Fig. 1B). BL/6 mice receiving the high fat diet had significantly elevated total body fat compared with their chow counterparts, whereas fat mass was slightly lower in the IIA+ groups regardless of the diet (Fig. 1C). We measured the caloric intake of the mice and found that over the 10 week feeding period the BL/6 and IIA+ mice consumed similar kilocalories of food per gram of body weight (Fig. 1D). Initially, the BL/6 mice on the high fat diet ate more kilocalories than the IIA+ mice, but after 8 weeks feeding was similar across all groups (data not shown). The unexpected observation was that the IIA+ mice on the high fat diet were protected from lipid accumulation, unlike the BL/6 mice on the high fat diet.

PLA2G2A and energy expenditure
Next, we assessed energy expenditure and physical activity using CLAMS. The BL/6 mice on the high fat diet had higher oxygen consumption than the chow-fed BL/6 mice ( Fig. 2A). Surprisingly, the IIA+ mice on chow and high fat diets had higher oxygen consumption than the BL/6 controls, suggesting increased energy expenditure regardless of diet. In addition to the elevated VO 2 consumption, the IIA+ mice had increased heat production (Fig. 2B). The BL/6 and IIA+ mice on the high fat diet had lower RER compared with their chow counterparts, indicating less carbohydrate utilization (Fig. 2C). The activity levels of the IIA+ mice were similar to the BL/6 mice on the same diets, indicating the increased energy expenditure was not due to increases in activity (Fig. 2D). However, the mice on the high fat diet were less active than those on the chow diet. Overall, the data in Figs. 1 and 2 suggest that the lean phenotype of the IIA+ mice was due to elevated basal metabolism.

PLA2G2A and insulin sensitivity
We examined the glucose and insulin tolerance of these mice after 10 weeks on the diets. Results from the GTT show that the IIA+ mice were able to clear glucose quite rapidly, even after 10 weeks on a high fat diet (Fig. 3A).

Fig. 2.
Metabolic analysis and activity of IIA+ mice on the high fat diet. After 10 weeks on the chow diet or HFD, mice were placed individually in CLAM chambers and their metabolism was assessed with respect to fat free mass (FFM). A: VO 2 consumption was measured as milliliters per hour of O 2 consumption over a 24 h period. B: Body heat production was expressed as calories per hour. C: RER was determined for all four groups of mice. D: Activity was determined by the number of beam breaks in both the light and dark cycles and is expressed as ambulatory movement. Each assessment is the average of measurements from 12 animals. Differences between groups were determined by two-way ANOVA, using genotype and diet as two separate factors. *P < 0.05, **P < 0.01, ***P < 0.001. However, the BL/6 mice on the high fat diet had reduced glucose clearance and higher levels of blood glucose 2 h after glucose administration compared with the BL/6 chow-fed mice. There was no effect of the high fat diet on glucose uptake in the IIA+ mice. In Fig. 3B, the total blood glucose amount over the 2 h assay is shown. To assess insulin sensitivity, we conducted an ITT. The BL/6 mice on the high fat diet had reduced insulin sensitivity and little change in blood glucose in response to insulin administration. In contrast, the IIA+ mice remained insulin sensitive regardless of the diet (Fig. 3C) and blood glucose remained low 2 h after insulin administration. The total change in blood glucose level over the 2 h assay is shown in Fig. 3D. These data suggest that PLA2G2A enhances whole-body insulin sensitivity.

Plasma lipid levels
Because previous studies have shown that the IIA+ mice have a propensity to develop atherosclerosis, we evaluated the blood lipids of the mice (17,20). The BL/6 mice on the high fat diet had significantly elevated serum cholesterol compared with all other groups. The IIA+ mice on the high fat diet had elevated cholesterol relative to the IIA+ chowfed group, but the overall cholesterol was lower in the IIA+ mice when compared with the BL/6 mice (Fig. 4A). Plasma triglycerides were lowest in the IIA+ chow animals (Fig. 4B). Triglycerides were highest in the IIA+ mice on the high fat diet, but this elevation was only significant when comparing them to their chow diet counterpart. HDL was greatly elevated in the BL/6 mice on high fat diet, and HDL levels were lower in the IIA+ groups relative to their dietary BL/6 controls (Fig. 4C). LDL was higher in the IIA+ mice on a high fat diet, although there was great variability among the animals (Fig. 4D). The increased LDL may reflect the higher triglycerides in the high fat-fed IIA+ mice. Because energy expenditure was higher in the IIA+ mice, we measured the T4 levels in the animals. There was no difference in the T4 between BL/6 and IIA+ mice, although the T4 was lower in both BL/6 and IIA+ animals on the high fat diet (Fig. 4E). Finally, we measured the triglyceride levels in The total blood glucose from all time points is shown. C: ITTs were conducted on all the mice in week 12 of the diet study, as described in the Materials and Methods. The blood glucose was measured at 15 min intervals following insulin injection. Each time point is the average of seven to nine mice. D: Total blood glucose for all time points in the ITT is shown. Differences in the glucose levels were determined by the t-test. *P < 0.05, **P < 0.01, ***P < 0.001. the liver. The hepatic triglycerides were greatly elevated in the BL/6 mice on the high fat diet, but the lipid levels in the IIA+ mice were only slightly increased (Fig. 4G).

Tissue expression of PLA2G2A
To determine where PLA2G2A was present, we tested various tissues for the expression of PLA2G2A. We observed PLA2G2A mRNA in liver, muscle, eWAT, and BAT ( Fig. 5A-D). The level of PLA2G2A mRNA in the liver was especially high. In the liver and muscle, PLA2G2A mRNA and protein abundance were increased by the high fat diet, indicating that the gene is stimulated by high fat feeding.

Metabolic gene expression in liver of IIA+ mice
To understand the molecular mechanisms underlying the metabolic effects of PLA2G2A, we measured the impact of PLA2G2A on the abundance of various mRNAs Fig. 4. Blood lipids in IIA+ mice. At the time of euthanization, serum was collected from the mice and blood lipids and thyroid hormone (T4) were measured at the University of Tennessee endocrinology laboratory. A: Cholesterol was measured as milligrams per deciliter of serum. B: Triglycerides were assessed as milligrams per deciliter of serum. C: HDLs were measured as milligrams per deciliter of serum. D: LDLs were measured as milligrams per deciliter of serum. E: T4s were measured as micrograms per deciliter of serum. F: Hepatic triglycerides were measured. Each point is the average of lipids from five animals. The data were analyzed by t-test. *P < 0.05, **P < 0.01, ***P < 0.001. encoding metabolic genes in the liver (Fig. 6). First, we measured the mRNA abundance of genes involved in fatty acid synthesis and oxidation, including Srebf1, Acaca, and carnitine palmitoyltransferase (Cpt1a). Acaca mRNA levels trended higher in the chow-fed IIA+ mice compared with the IIA+ mice on high fat diet (P = 0.2871), but the levels were not significant. (Fig. 6A). Surprisingly, the Srebf1 mRNA was reduced in the IIA+ mice (Fig. 6B). Cpt1a was elevated in response to the high fat diet in BL/6 mice, but not in the IIA+ mice (Fig. 6C). Next, we measured the hepatic abundance of two genes important for mitochondrial biogenesis, Ppargc1a and Sirt1. The mRNA levels of Ppargc1a and Sirt1 were higher in the IIA+ mice on the chow diet, but not the high fat diet (Fig. 6D, E). To identify changes in protein levels, we measured the abundance of hepatic proteins involved in metabolic regulation by Western analyses. The abundance of ACC1 was elevated in both the BL/6 and IIA+ mice on high fat diet (Fig. 7A). The precursor SREBP-1c protein was elevated in the BL/6 mice in response to the high fat diet, while nuclear SREBP-1c was elevated in the IIA+ mice on the high fat diet (Fig. 7B, C). Because the IIA+ mice had elevated energy expenditure, we examined inducers of mitochondrial biogenesis, including PPAR coactivator 1 (PGC-1) and SIRT1. We did not see any increase in either PGC-1 or SIRT1 protein in the liver, suggesting that these factors, at least in the liver, do not promote hepatic mitochondrial biogenesis (Fig. 7D, E).
Because the IIA+ mice are more insulin sensitive, we tested to determine whether kinases in the insulin signaling pathway were activated in the IIA+ mice. There was increased Akt in the IIA+ mice and there was elevated activation of Akt based on the phospho-Akt(T308) to Akt ratio in the IIA+ mice both on the chow and on the high fat diet (Fig. 8A, C). In addition, we examined S6K, which is downstream of mTORC1. Our data show that there was increased abundance of S6 kinase and phospho-S6 kinase(Thr389), particularly in the IIA+ mice (Fig. 8B, C). These data suggest that there is enhanced insulin signaling in the IIA+ mice.
To determine whether there were changes in other tissues that might account for the increased energy expenditure and heat production in the IIA+ mice, we measured mRNA and protein levels of UCP1, PGC-1, and SIRT1 in eWAT, BAT, and muscle. We did not detect differences in PGC-1 and SIRT1 abundance in muscle (data not shown). In eWAT, there was very little gene or protein expression of these metabolic proteins (data not shown). However, in the BAT, Ucp1, Ppargc1a, and Sirt1, mRNA levels were greatly increased (Fig. 9A). We measured the levels of these proteins as well and found that all three were elevated (Fig. 9B). These data suggest that part of the activation of the metabolic rate comes from increased mitochondrial uncoupling and, thus, increased energy dissipation as heat from BAT.

DISCUSSION
Elevated levels of the PLA2G2A are associated with inflammation and cardiovascular disease (8,31). Here, we investigated the metabolic response of IIA+ mice to a high fat diet. Our data indicate that PLA2G2A provided protection from the deleterious effects of high fat diets. First, the IIA+ mice did not gain weight or accumulate fat mass in response to the high fat diet and, additionally, remained insulin sensitive and glucose tolerant. Second, the IIA+ mice had elevated energy expenditure and heat generation, likely due to the induction of mitochondrial uncoupling in BAT. Our results demonstrate that PLA2G2A enhances insulin responsiveness and overall energy utilization.
The focus of many previous studies on PLA2G2A has been on the regulation of cholesterol levels and the impact of PLA2G2A on atherogenesis (20,21,32). sPLA 2 enzymes, including PLA2G2A, PLA2G3, and PLA2G5, are proatherogenic due to their ability to promote the oxidation of LDL and HDL particles and to increase the formation of lipid-laden foam cells from macrophages (5). Introduction of PLA2G2A-expressing bone marrow cells into Ldlr / mice increased the formation of atherosclerotic lesions (21).
Likewise, PLA2G3 has been found to enhance the development of atherosclerosis and small pro-atherogenic LDL particles (33). PLA2G2A promotes atherogenesis in IIA+ mice fed a high cholesterol diet (17) and IIA+ mice had hepatic cholesterol accumulation on an atherogenic diet (19). Our experimental design differed from previous studies in that our diets contained significantly greater amounts of fat, but less cholesterol. Consistent with the mice on atherogenic diets, we found that there was reduced blood cholesterol in our IIA+ mice (Fig. 4A). Our chow-fed IIA+ mice also had lower HDL and reduced serum triglycerides. The lower HDL raises the possibility that the mice have diminished reverse cholesterol transport. The elevated triglycerides in the IIA+ mice on the high fat diet are likely associated with increased LDL particles (Fig. 4D). The lower HDL and elevated LDL may contribute to the atherosclerosis observed in the IIA+ mice (17). A trend toward elevated VLDL/LDL particles was previously reported in IIA+ mice on an atherogenic diet (17). The IIA+ mice also had reduced Cpt1a mRNA, suggesting less hepatic fatty acid oxidation (Fig. 6C). In addition, the IIA+ mice had higher levels of ACC1 and nuclear SREBP-1c, which may also contribute to the hyperlipidemia (Fig. 7). Overall, our data are consistent with previous observations of changes in blood lipids in the IIA+ mice.
An unexpected finding of our experiments was that the IIA+ mice did not gain weight or accumulate fat mass on the high fat diet. Only the BL/6 mice on the high fat diet accumulated fat. The IIA+ mice were smaller than the BL/6 mice and consumed similar kilocalories per gram of body weight over the 10 week period. The increased energy expenditure was likely the major cause of the reduced weight gain by the IIA+ mice. Increased energy expenditure was not due to increased activity in the IIA+ mice, as they were not more active than the BL/6 mice (Fig. 2D). Elevated thyroid hormone concentrations were not responsible (Fig. 4E). In the liver, key regulators of proteins involved in fatty acid oxidation, such as PGC-1 and SIRT1, were not elevated. The transcriptional events that specify a brown adipogenic program have been well-characterized. PGC-1 and the transcription factor PR domain containing 16 (PDRM16) directly regulate BAT induction, and SIRT1 may promote adipose tissue browning as well (34)(35)(36). We observed increased expression of UCP1, PGC-1, and SIRT1 in the BAT of the IIA+ mice, suggesting that PLA2G2A promotes mitochondrial uncoupling in BAT (Fig. 9).
It was previously discovered that lipoproteins modulated by sPLA 2 were more susceptible to lipid peroxidation, resulting in the production of oxidized lipoproteins and the byproduct, 4-hydroxy-2-nonenal (4-HNE). The 4-HNE can induce mitochondrial uncoupling through covalent modification of UCPs and adenine nucleotide translocase (ANT), an ATP exporter (37). Given the increases we have observed in overall brown adipogenic programming through induction of PGC-1 and SIRT1, as well as induction of UCP1 itself, in the IIA+ mice, a possible explanation is that overexpression of PLA2G2A enhanced lipid peroxidation and, therefore, 4-HNE. The 4-HNE could activate mitochondrial uncoupling through its stimulatory actions on proton conductance.
One of the many fatty acids released by sPLA 2 activity is arachidonic acid, a necessary precursor for biosynthesis of a diverse group of eicosanoids and other signaling molecules involved in the inflammatory response (38). However, it was recently discovered that arachidonic acid alone can also directly activate the mitochondrial proton leak caused by UCP1 in both beige and brown adipocytes through experiments using inner mitochondrial matrix mitoplasts (39). Moreover, a similar study found that UCP1 can be controlled by fatty acid products generated from phospholipid hydrolysis by cytosolic PLA 2 (40). The IIA+ mice on chow and high fat diets had elevated VO 2 ( Fig. 2A) and heat dissipation (Fig. 2B). This may be due to enhanced release of arachidonic acid or other long chain fatty acid (LCFA) signaling molecules by sPLA 2. This LCFA then migrates to BAT and induces mitochondrial uncoupling through UCP1's LCFA anion/H + symporter activity.
Because PLA2G2A is frequently associated with inflammation and rheumatoid disease, we anticipated that the IIA+ mice would be insulin resistant, as inflammation has been frequently linked with insulin resistance (41,42). However, another unexpected observation was that the IIA+ mice were much more sensitive to insulin, as demonstrated by both the GTT and the ITT (Fig. 3). Furthermore, the hepatic insulin signaling pathways were clearly activated, as shown by the increased abundance and phosphorylation of Akt (Fig. 8). One possibility is that as the IIA+ mice were refractory to high fat diet-induced obesity, the insulin sensitivity was secondary to the different obesity states. Previous work found that cholesterol accumulated in the liver of IIA+ mice on an atherogenic diet (19). The livers of the BL/6 mice on high fat diet contained much more triglyceride than those of all other groups (Fig. 4G). We observed a slight increase in hepatic triglycerides in the IIA+ mice on high fat diet compared with the chow groups. The attenuated accumulation of triglycerides in the IIA+ mice may provide an additional Recent studies have found that several sPLA 2 isoforms possess different and tissue-specific roles in obesity and metabolic dysfunction. Hui and colleagues reported that the knockout of PLA2g1b provided protection against dietinduced obesity and insulin resistance (43). PLA2g1b is secreted into the intestinal lumen from the pancreas and the protection arose from reduced lysophosphatidylcholine absorption. The PLA2g1b knockout mice also had elevated hepatic fatty acid oxidation, indicating that the livers relied more on fatty acids for energy (44,45). Addition of lysophosphatidylcholine to isolated hepatocytes directly reduced mitochondrial oxidation of fatty acids (46). Work from Murakami and colleagues examined the interplay of PLA2g5 and PLA2g2e in the adipose tissue with respect to metabolic actions (47). They found that PLA2g5 knockout mice displayed insulin resistance and hyperlipidemia, and were increasingly prone to diet-induced obesity, suggesting that PLA2g5 provides protection from obesity. In the same study, it was also discovered that knocking out PLA2g2e in mice decreased hyperlipidemia and diet-induced obesity, and improved overall liver health, indicating that PLA2g2e may promote obesity (47). A metabolic role for PLA2g2a was investigated in rats (48). With a high fat diet, the levels of PLA2g2a were elevated 20-fold in rat white adipose tissue (WAT) (48). In concurrence with these studies, we previously reported that BALB/c mice on a high fat diet had increased PLA2g2a mRNA in the liver and that the addition of oleic acid to rat hepatocytes increased PLA2g2a (25). In rats, chronic administration by gavage of the PLA2g2a inhibitor, KH064, protected against diet induced weight gain and reduced fasting glucose concentrations (48). The authors proposed that PLA2g2a inhibition improved WAT function via stimulating lipolysis, thereby decreasing fat stores in WAT (48). These results differ from our observations that elevated PLA2G2A activity enhances energy expenditure in BAT and overall insulin sensitivity. It may be that the results in the rat study stem from an impairment of intestinal lipid absorption, thereby reducing the impact of the high fat diet, as Iyer et al. (48) observed increased fat in the feces of the KH064 treated rats. It is clear from multiple studies that the various secretory phospholipases can have isoformspecific and tissue-specific effects. For example, Boilard et al. (15) reported that PLA2g5 has an anti-inflammatory role that counteracts the inflammation mediated by PLA2g2a in rheumatoid arthritis. Therefore, the role of PLA2G2A and other sPLA 2 isoforms in metabolism requires further investigation.
In summary, we have found that PLA2G2A improves insulin sensitivity and provides resistance to high fat Fig. 8. Alterations in insulin signaling pathways in IIA+ mice. Proteins were collected from the livers at the end of the feeding period and the abundance of various kinases was analyzed by Western blot. Kinase abundance was measured and the ratio of phospho-kinase to kinase is shown. pAkt/Akt (A), pS6K/S6K (B), representative Western blots are shown (C). Each point is the average of proteins from four animals. The data were analyzed by t-test. *P < 0.05, **P < 0.01. diet-induced weight gain in mice. Furthermore, PLA2G2A increases the energy expenditure of the mice independent of activity and diet, partly through UCP1 and induction of BAT. We have identified a previously undescribed role of this secretory phospholipase in the modulation of BAT and metabolism. Future studies will determine the mechanisms underlying this phenomenon. Fig. 9. Increased mitochondrial uncoupling in IIA+ mice on chow-fed diet. RNA and proteins were collected from the BAT of BL/6 and IIA+ mice after a 10 week feeding period. A: The relative mRNA levels of Ucp1, Sirt1, and Ppargc1a are shown. B: The relative protein abundance of UCP1, SIRT1, and PGC-1 are shown. C: The Western blots from the BAT are shown. Each point is the average of mRNA/protein from four animals. Data were analyzed by t-test. *P < 0.05, **P < 0.01, ***P < 0.001.