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COVER: Activation of cytosolic phospholipase A₂ (cPLA₂) by 25-hydroxycholesterol in CHO-K1 cells. cPLA₂ is activated by increases in cellular Ca²⁺ concentration through relocalization to the nuclear envelope. Cells were examined, by fluorescence microscopy and digital deconvolution, after transient expression of a green fluorescent protein-cPLA₂ fusion protein for 48 h, followed by an additional 6 h incubation in the absence (left) or presence (right) of 3 &mgr;g/ml 25-hydroxycholesterol. (See Panini et al., p. 1678.)
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