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COVER: Intracellular shift of 12-NBD stearate. Left: A HepG2 cell after 10 min incubation with 12-NBD stearate. Note that after prolonged incubation a significant portion of 12-NBD stearate associates with larger organelles preferentially situated in the perinuclear region. In double-labeling experiments with 12-NBD stearate (left) and Mitochondrial Tracker fluorescent dye (middle) a main portion of these perinuclear structures can be identified as mitochondria. Merged images (right): Locations of colocalization resulting in yellow fluorescence. Arrowhead: Some remaining small vesicles next to plasma membrane staining positive for 12-NBD stearate (visualization by an upright Zeiss LSM 310 system). (See Pohl, Ring, and Stremmel, p.1390.)
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