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Intracellular distribution of NBD-cholesterol and lysosomes in adipocytes.
Cells were incubated in DMEM in presence of HDL-NBD-cholesterol and LysoTracker
Red for 1 h at 37
C.
Top Left: LysoTracker labeling of lysosomes.
Bottom left: NBD-cholesterol labeling of lipid droplets.
Top right: transmitted light image obtained with a Zeiss laser scanning microscope.
Bottom right: merged images. Bar =
m.
(See Dagher et al., p.1811.)
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Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.