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COVER: Filipin staining for free cholesterol (in red), and immunofluorescence staining for the lysosome associated membrane protein LAMP-1 (in green), in mouse embryonic fibroblasts deficient in both mannose 6-phosphate receptors. The accumulation of free cholesterol in lysosomal vesicles is due to mistargeting of the NPC2 protein, which is needed for transport of cholesterol out of lysosomes. (See Willenborg et al., p. 2559.)
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