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COVER: High-density lipoprotein particles containing wild-type and mutant L159R apoA-I (apoA-I_Fin) molecules can be quantified using a combination of ELISA and mass spectrometry. The tryptic peptide AHVDAL ¹⁵⁹R (green, wild-type apoA-I) can be distinguished from AHVDAR¹⁵⁹ (red, L159R apoA-I) after separation of mouse plasma on SDS-PAGE followed by in-gel trypsin digest and HPLC-electrospray mass spectrometry (LC-MS). (See Owen et al., p. 226.)
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