Abstract
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Harel, M., Tandler, A., Kissil, G. W., Applebaum, S. W., 1995. The role of broodstock dietary protein in vitellogenin synthesis and oocyte development, and its effects on reproductive performance and egg quality in gilthead seabream Sparus auratus, . In Proceedings of Fifth Int. Symp. Reprod. Physiol. Fish. Goetz, F. W., Thomas, P., editors. Fish Symposium Austin 95, Austin, TX. 105–107.
MATERIALS AND METHODS
Preparation of mRNA and identification of rainbow trout VTG receptor cDNA by RT-PCR
Construction and screening of a cDNA library
Expression of rainbow trout VTGR in COS-1 cells and in vitro translation
Ligand preparation, electrophoresis and ligand blotting
Northern blot analysis
In situ hybridization
Probe synthesis
RESULTS
Cloning of the trout oocyte VTGR



In vitro translation and expression of the trout VTGR in COS-1 cells

Localization of VTGR mRNA in ovarian follicles

DISCUSSION
Acknowledgments
References
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