MATERIALS AND METHODS
Chemicals and antibodies
Cell culture
Plasmids and transfection
RNA interference
Immunoprecipitation
TAG measurement
Immunofluorescence microscopy and image analysis
Electron microscopy
Fluorescence recovery after photobleaching
Differentiation of 3T3-L1 cells and measurement of intensity of Oil Red O staining
Proximity ligation assay
Subcellular fractionation
Statistical analyses
RESULTS
Stx17 is required for LD biogenesis


Depletion of Stx17 causes aberrant distribution of ACSL3 on the LD surface

Stx17 regulates the redistribution of ACSL3 to LDs


Binding between Stx17 and ACSL3

Transient overexpression of ACSL3 can compensate for Stx17 depletion

SNAP23 can outcompete the ACSL3 -Stx17 interaction

DISCUSSION

Acknowledgments
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Footnotes
This work was supported in part by Ministry of Education, Culture, Sports, Science and TechnologyGrants-in-Aid for Scientific Research25291029 and 26650066(to M.T.), and 26111520and26713016(to K.A.), and the Ministry of Education, Culture, Sports, Science and Technology-supported program for the Strategic Research Foundation at Private Universities (to M.T. and K.A.). The authors declare that they have no conflicts of interest with the contents of this article.
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