Advertisement
Rapid Communications| Volume 38, ISSUE 7, P1490-1495, July 1997

Alternative forms of the scavenger receptor BI (SR-BI)

Open AccessPublished:July 01, 1997DOI:https://doi.org/10.1016/S0022-2275(20)37431-9
      This paper is only available as a PDF. To read, Please Download here.
      The class B, type I scavenger receptor has been implicated as a receptor for high density lipoprotein (HDL). We have isolated a murine cDNA clone encoding an alternative form of SR-BI that differs in the putative cytoplasmic domain of the receptor. This variant form, likely the result of alternative mRNA splicing, is designated SR-BI.2. SR-BI.2 mRNA was detected in mouse tissues known to express SR-BI and tissue-specific differences in the relative abundance of SR-BI.2 were apparent. In mouse adrenal glands, SR-BI.2 represented approximately one-third of total SR-BI mRNA, whereas in mouse testes, SR-BI.2 represented the major mRNA species (79% of total). SR-BI.2 was also detected in the human cell lines examined, namely HeLa, HepG2, and THP-1 cells. CHO cells transfected with the mouse SR-BI.2 cDNA expressed significant levels of SR-BI.2 protein and acquired the ability to take up fluorescent lipid (DiI) from DiI-HDL. Alternative splicing of SR-BI represents a potentially important process for the regulation of SR-BI expression and function.

      REFERENCES

        • Gordon D.J.
        • Rifkind B.M.
        High-density lipoprotein the clinical implications of recent studies.
        N. Engl. J. Med. 1989; 321: 1311-1316
        • Acton S.
        • Rigotti A.
        • Landschulz K.T.
        • Xu S.
        • Hobbs H.H.
        • Krieger M.
        Identification of scavenger receptor SR-BI as a?high density lipoprotein receptor.
        Science. 1996; 271: 518-520
        • Landschulz, K T.
        • Pathak R.K.
        • Rigotti A.
        • Krieger M.
        • Hobbs H.H.
        Regulation of scavenger receptor, class B, type I, a high density lipoprotein receptor, in liver and steroidogenic tissues of the rat.
        J. Clin. Invest. 1996; 98: 984-995
        • Rigotti A.
        • Edelman E.R.
        • Seifert P.
        • Iqbal S.N.
        • DeMattos R.B.
        • Temel R.E.
        • Krieger M.
        • Williams D.L.
        Regulation by adrenocorticotropic hormone of the in vivo expression of scavenger receptor class B type I (SR-BI), a high density lipoprotein receptor, in steroidogenic cells of the murine adrenal gland.
        J. Biol. Chem. 1996; 271: 33545-33549
        • Wang N.
        • Weng W.
        • Breslow J.L.
        • Tall A.R.
        Scavenger receptor BI (SR-BI) is up-regulated in adrenal gland in apolipoprotein A-I and hepatic lipase knock-out mice as a response to depletion of cholesterol stores.
        J. Biol. Chem. 1996; 271: 21001-21004
        • Lee D.C.
        • Rose T.M.
        • Webb N.R.
        • Todaro G.J.
        Cloning and sequence analysis of a cDNA for rat transforming growth factor-alpha.
        Nature. 1985; 313: 489-491
        • Altschul S.F.
        • Gish W.
        • Miller W.
        • Myers E.W.
        • Lipman D.
        Basic local alignment search tool.
        J. Mol. Biol. 1990; 215: 403-410
        • Andersson S.
        • Davis D.N.
        • Dahlback H.
        • Jornvall H.
        • Russell D.W.
        Cloning, structure and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme.
        J. Biol. Chem. 1989; 264: 8222-8229
        • Kingsley D.M.
        • Krieger M.
        Receptor-mediated endocytosis of low density lipoprotein: somatic cell mutants define multiple genes required for expression of sur-face-receptor activity.
        Proc. Natl Acad. Sci. USA. 1984; 81: 5454-5458
        • Pitas R.E.
        • Innerarity T.L.
        • Weinstein J.N.
        • Mahley R.W.
        Acetoacetylated lipoproteins used to distinguish fibroblasts from macrophages in vitro by fluorescence microscopy.
        Arteriosclerosis. 1981; 1: 177-186
        • Geng Y-J.
        • Hansson G.K.
        Interferon-y inhibits scavenger receptor expression and foam cell formation in human monocyte-derived macrophages.
        J. Clin. Invest. 1992; 89: 1322-1330
        • Peterson M.L.
        • Perry R.P.
        The regulated production of μm and μs mRNA is dependent on the relative efficiencies of μs poly (A) site usage and the Cμ4-to-M1 splice.
        Mol. Cell. Biol. 1989; 9: 726-738
        • Calvo D.
        • Vega M.A.
        Identification, primary structure, and distribution of CLA-1, a novel member of the CD36/LIMPII gene family.
        J. Biol. Chem. 1993; 268: 18929-18935
        • Acton S.L.
        • Scherer P.E.
        • Lodish H.F.
        • Krieger M.
        Expression cloning of SR-BI, a CD36-related class B scavenger receptor.
        J. Biol. Chem. 1994; 269: 21003-21009
        • Vega M.A.
        • Segui-Real B.
        • Garcia J.A.
        • Cales C.
        • Rodriguez F.
        • Vanderkerckhove J.
        • Sandoval I.V.
        Cloning, sequencing, and expression of a cDNA encoding rat LIMP II, a novel 74-kDa lysosomal membrane protein related to the surface adhesion protein CD36.
        J. Biol. Chem. 1991; 266: 16818-16824
        • Armesilla A.L.
        • Vega M.A.
        Structural organization of the gene for human CD36 glycoprotein.
        J. Biol. Chem. 1994; 269: 18985-18991
        • Noguchi K.
        • Naito M.
        • Tezuka K.
        • Ishii S.
        • Seimiya H.
        • Sugimoto Y.
        • Amann E.
        • Tsuruo T.
        cDNA expression cloning of the 85-kDa protein overexpressed in adria-mycin-resistant cells.
        Biochem. Biophys. Res. Commun. 1993; 192: 88-95
        • Horowitz D.S.
        • Krainer A.R.
        Mechanisms for selecting 5'-splice sites in mammalian pre-mRNA splicing.
        Trends Genet. 1994; 10: 100-106
        • Huang M-M.
        • Bolen J.B.
        • Barnwell J.W.
        • Shattil S.J.
        • Brugge J.S.
        Membrane glycoprotein IV (CD36) is physically associated with the Fyn, Lyc, and Yes protein-tyrosine kinases in human platelets.
        Proc. Natl. Acad. Sci. USA. 1991; 88: 7844-7848