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ANGPTL4 inactivates lipoprotein lipase by catalyzing the irreversible unfolding of LPL's hydrolase domain

Open AccessPublished:April 23, 2020DOI:https://doi.org/10.1194/jlr.ILR120000780
      EQUIPMENT: UPLC-ESI-MS with a quadrupole time-of-flight mass spectrometer (Synapt G2; Waters).
      REAGENTS: Purified bovine LPL from fresh milk, a soluble human GPIHBP1 (residues 1-131) expressed in Drosophila S2 cells, and a truncated version of ANGPTL4 (1-159) expressed in Escherichia coli

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