December 2007
Volume 48Issue 12p2529-2795
Open Access
COVER: Peritoneal macrophages isolated from wild-type mice (top and middle left panels)
and mice deficient in CD36 (bottom and middle right panels) were incubated with Alexa568-oxidized
LDL (red fluorescence) and then immunostained to detect dynamin, a GTPase involved
in cellular membrane fission events during the formation of endocytic vesicles (green
fluorescence). Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue).
The colocalization of intracellular oxidized LDL and dynamin in macrophages (yellow)
is CD36 dependent. This novel finding provides evidence that different endocytic mechanisms
participate in macrophage uptake of oxidized LDL. Molecular sorting of oxidized LDL
at the plasma membrane may lead to differences in subsequent intracellular trafficking
and degradation. (See Sun et al., p. 2560.)...Show more
COVER: Peritoneal macrophages isolated from wild-type mice (top and middle left panels)
and mice deficient in CD36 (bottom and middle right panels) were incubated with Alexa568-oxidized
LDL (red fluorescence) and then immunostained to detect dynamin, a GTPase involved
in cellular membrane fission events during the formation of endocytic vesicles (green
fluorescence). Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue).
The colocalization of intracellular oxidized LDL and dynamin in macrophages (yellow)
is CD36 dependent. This novel finding provides evidence that different endocytic mechanisms
participate in macrophage uptake of oxidized LDL. Molecular sorting of oxidized LDL
at the plasma membrane may lead to differences in subsequent intracellular trafficking
and degradation. (See Sun et al., p. 2560.)
